Effects of Huanghou Zhixie Dropping Pill on intestinal mucosal barrier and intestinal flora in mice with ulcerative colitis
Objective:To evaluate the effects of Huanghou Zhixie Dropping Pill on intestinal mucosal barrier and intestinal flora in mice with ulcerative colitis.Methods:Balb/c mice were randomly divided into blank group,model group,Huanghou low-dose group,Huanghou medium-dose group,Huanghou high-dose group and mesala-zine control group,with 6 mice in each group.Except for the blank group,the other groups were molded,and af-ter successful modeling,the mice were fed with drugs of the corresponding group.2 weeks later,the colon of the mice was removed and feces near the anus were collected for freezing storage.Western blot was used to detect compact nexin and 16sRNA was used to detect fecal flora and comparative analysis was performed.Results:Com-pared with blank group,the expression levels of tight junction proteins claudin-1,ZO-1 and occludin in model group were significantly decreased(P＜0.01),and the contents of tight junction proteins claudin-1,ZO-1 and oc-cludin in Huanghou Zhixie Dropping Pill group were significantly increased(P＜0.05).Further comparison showed that the effect of Huanghou medium dose group was good.α diversity and species difference analysis showed that there were significant differences in the composition of microflora among all groups.Further analysis showed that Bacteroidetes decreased and Proteobacteria increased in the intestinal tracts of mice in the model group.β diversity analysis,species composition heat map and LefSe analysis showed that the species in the three groups were significantly different.According to ZIPI map,the five key species with the highest abundance in each group were Firmicutes,Bacteroidetes,Proteobacteria,soficutes and verrucobacteria,respectively.Function-al potential prediction found that the core flora mainly affected the synthesis of amino acids,nucleotides,repair groups,electron carriers and vitamins,but also affected the degradation of carbohydrates,carboxylate,nucleo-sides and nucleotides.Conclusion:Huanghou Zhixie Dropping Pill can increase the content of claudin-1,occludin and ZO-1 in colonic tissue of ulcerative colitis mice,and improve the intestinal permeability of ulcerative colitis mice.Promote the growth of probiotics and symbiotic bacteria,inhibit the colonization of opportunistic pathogens or harmful bacteria,and maintain the homeostasis of intestinal flora;Ulcerative colitis is treated by altering the number of core microflora to affect biological processes such as synthesis and degradation.
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