Effect of spleen deficiency syndrome on intestinal uric acid excretion in hyperuricemia mice
Objective:To explore the mechanism of the effect of spleen deficiency syndrome on uric acid me-tabolism from the blood uric acid level,fecal uric acid level,intestinal morphological changes,and the expression of intestinal uric acid transporter ABCG2 and GLUT9.Methods:Mice were randomly divided into blank group,spleen deficiency group,HUA group and spleen deficiency HUA group.The spleen deficiency syndrome model was made with compound spleen injury factors,and the HUA model was made with 2％uric acid+3％potassium oxazinate feed.Anal temperature,body weight and diet of mice were monitored during spleen deficiency model-ing.Blood samples were collected to detect uric acid and urea nitrogen,stool was collected to detect fecal uric acid level.The tissues of jejunum,ileum and colon were stained with Hematoxylin Eosin staining(HE)and the protein expression of ABCG2 and GLUT9 were detected by Western Blot.Results:Evaluation of spleen deficiency model:compared with normal mice,the weight of spleen deficiency mice was decreased(P＜0.05),the diet was signifi-cantly decreased(P＜0.01),and the anal temperature was decreased(P＜0.05).The body weight of mice in the spleen deficiency HUA group increased gradually during the HUA modeling,and there was no significantdiffer-ence between the two group at the end of the modeling.The blood uric acid level of mice in spleen deficiency HUA group was higher than that in HUA group(P＜0.05),but there were no significant differences in urea nitrogen and creatinine levels among the four groups.The fecal uric acid of the spleen deficiency HUA group was signifi-cantly lower than that of HUA group(P＜0.05).HE staining showed that the villi of ileum and colon tissues were shortened and loosely arranged in the spleen deficiency group,while the villi of jejunum and ileum tissues were loosely arranged in the spleen deficiency HUA group,and goblet cells of colon tissues were reduced.West-ern Blot analysis showed that the expression of ABCG2 and GLUT9 decreased in colon tissues of spleen deficiency HUA group.IHC analysis showed that the expression of ABCG2 and GLUT9 increased in colon tissues of HUA group compared to blank group,and the expression of ABCG2 and GLUT9 decreased in colon tissues of spleen de-ficiency HUA group compared to HUA group.Conclusion:Intestinal uric acid excretion disorder in hyperuricemia mice with spleen deficiency syndrome leads to increased blood uric acid level,which may be related to the de-creased expression of intestinal uric acid transporters ABCG2 and GLUT9.
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