Effects of total paeony glucosides on proliferation,apoptosis and migration of colorectal cancer SW-480 cells by regulating miR-338-3p/GMFB expression
Objective:To investigate the effects of total paeony glucosides(TPG)on proliferation,apoptosis and migration of colorectal cancer cells by regulating miR-338-3p/glia maturation factor β(GMFB)expression.Methods:Human colorectal cancer SW-480 cell line was cultured in vitro and treated with different concentrations of TPG.Cell proliferation was detected by plate cloning assay,cell apoptosis was detected by flow cytometry,cell migration was detected by scratch assay,and cell invasion was detected by Trans well assay.The expression of miR-338-3p and GMFB in cells was detected by qRT-PCR,and the targeting relationship between miR-338-3p and GMFB was detected by dual luciferase reporter gene.Results:Compared with the control group,the number of clone formation,cell healing rate,and migration in the low-dose,medium dose,and high-dose TPG groups were significantly reduced(P＜0.001),the high-dose TPG group showed the most significant reduction in clone forma-tion,cell healing rate,and migration number;compared with the control group,the apoptosis rate of cells in the low-dose,medium dose,and high-dose TPG groups significantly increased(P＜0.001).the high-dose TPG group showed the most significant increase in cell apoptosis rate;compared with the control group,the expression of miR-338-3p was significantly increased in the low-dose,medium dose,and high-dose TPG groups(P＜0.001),the expression of miR-338-3p was most significantly increased in the high-dose TPG group;compared with the control group,the GMFB mRNA expression in the low-dose,medium dose,and high-dose TPG groups was sig-nificantly reduced(P＜0.001),the GMFB mRNA expression was most significantly reduced in the high-dose TPG group;compared with the miR-con group,the number of clone formation,cell healing rate,and migration in the anti-miR-338-3p group significantly increased,while the apoptosis rate was significantly reduced(P＜0.001);miR-338-3p significantly reduced the luciferase activity of GMFB-WT(P＜0.05),but had no effect on the lucifer-ase activity of GMFB-MUT(P＞0.05);compared with high dose TPG+miR-con,high dose TPG+anti-miR-338-3p significantly increased the number of clone formation,cell healing rate,and migration,while significantly decreased the apoptosis rate(P＜0.001).Conclusion:TPG may inhibit the proliferation,apoptosis and migration of colorectal cancer cells by regulating the miR-338-3p/GMFB.
万方数据
维普
