Effect of Yu's Qingrehuoxue formula to ulcerative colitis rats'colon mucosa on the expression of NLRP3,IL-1β and NF-κB
Objective:This study was done to study Yu's Qingrehuoxue formula's effect to ulcerative colitis rats'colon mucosa on the expression of NLRP3,IL-1β and NF-κB,to prove that Yu's formula can reduce inflammatory response.Methods;Thirty Wistar rats were randomly divided into six groups,include control group,model group,mesalazine group,Chinese medicine high-dose,medium-dose and low-dose groups(5 in each group).After successful modeling with TNBS(trinitrobenzene sulfonic acid),the rats were given 14 days enema with distilled water,high,medium and low concentration Yu's Qingrehuoxue formula and mesalazine suspension.Use Western blot and immunohistochemistry to test the expression of NLRP3,IL-1β and NF-κB in colon mucosa in every group.Results:Western blot and immunohistochemistry results all showed that compared with control group,the expression levels of NLRP3,IL-1β and NF-κB protein in colon tissue of model group were significantly increased(P＜0.01).Compared with model group,the expression levels of NLRP3,IL-1β and NF-κB protein in colon tis-sues of mesalazine,high-dose,medium-dose and low-dose groups were significantly decreased(P＜0.05 or P＜0.01).Conclusion:Yu's Qingrehuoxue formula maybe through reducing the expression of NLRP3,IL-1β and NF-κB to reduce the inflammation in UC.
Yu's Qingrehuoxue formulaulcerative colitistraditional Chinese medicine enemaNOD-like re-ceptor thermal protein domain associated protein 3
万方数据
