Analysis of different colonic transport dynamics in patients with functional constipation based on 16S sequencing technology differential study of intestinal flora in patients with functional constipation
Objective:To analyze the differences in intestinal flora between patients with functional constipa-tion(FC)of different colonic transit times and a normal group using 16S rDNA sequencing technology.Methods:Sixty FC patients from the Second People's Hospital affiliated with Fujian University of Traditional Chinese Medi-cine were recruited and divided into two groups based on the results of colonic transit tests:20 cases in the delayed colonic transit time(DCTT)group and 40 cases in the normal colonic transit time(NCTT)group.Additionally,30 healthy volunteers were recruited as a normal control group.Fresh feces were collected from each group,and 16S rDNA sequencing was used to analyze differences in gut microbiota.The sequencing results were subjected to gene function prediction.Results:(1)α diversity analysis:The abundance indices Chao1 and observed_otus in the DCTT group were higher than those in the NCTT group and the normal control group(P＜0.05).There were no statistically significant differences in diversity(Shannon index,Simpson index)among the three groups(all P＞0.05).(2)Community Composition Analysis:The predominant phyla in all three groups were Firmicutes,Bacte-roidetes,and Proteobacteria,with Bacteroides and Faecalibacterium being the dominant genera.At the genus lev-el,Blautia,Roseburia,Agathobacter,Klebsiella,Fusicatenibacter,Ruminococcus_gnavus_group,Romboutsia,Eubacterium_hallii_group,Dorea,and Lachnospiraceae_ND3007_group were decreased in both the DCTT and NCTT groups compared to the normal control group(P＜0.05),but there were no significant differences between the DCTT and NCTT groups(P＞0.05).Genera such as Dialister,Lactobacillus,UCG_002,Lachnoclostridium,Christensenellaceae_R_7_group,and Family_ⅩⅢ_AD3011_group were increased in the DCTT and NCTT groups compared to the normal control group(P＜0.05),with no significant differences between the DCTT and NCTT groups(P＞0.05).Genera such as Parabacteroides,Subdoligranulum,and Flavonifractor were increased in the NCTT group compared to the normal control group(P＜0.05),with no significant differences compared to the DCTT group(P＞0.05).Butyricimonas were increased in the DCTT group compared to the normal control group(P＜0.05),with no significant differences compared to the NCTT group(P＞0.05).Parasutterella was de-creased in the DCTT group compared to the normal control group(P＜0.05),with no significant differences com-pared to the NCTT group(P＞0.05).(3)LEfSe Analysis:The normal control group had Blautia,Roseburia,Agathobacter,Escherichia-Shigella,Eubacterium coprostanoligenes group,Anaerostipes,Intestinimonas,Fusi-catenibacter,Ruminococcus_gnavus_group,Romboutsia,and Klebsiella as biomarkers.The DCTT group had Lactobacillus,UCG_002,Alistipes,and Clostridia_UCG_014 as biomarkers.The NCTT group had Dialister,Subdoligranulum,Parabacteroides,and Christensenellaceae_R_7_group as biomarkers.(4)Functional Prediction Analysis:The LYCOCAT-PWY and TRPSYN-PWY pathways were decreased in the DCTT group compared to the normal control group(P＜0.05),with no significant differences between the NCTT group and the normal con-trol group(P＞0.05).The PWY-621 pathway was decreased in both the DCTT and NCTT groups compared to the normal control group(P＜0.05).Pathways such as PWY-6317,PWY-5384,FUC-RHAMCAT-PWY,P461-PWY,PWY-6629,and P341-PWY were decreased in both the DCTT and NCTT groups compared to the normal control group(P＜0.05).The PWY-7254 pathway was increased in the NCTT group compared to the normal control group(P＜0.05),with no significant differences between the DCTT group and the normal control group(P＞0.05),suggesting that gut microbiota in FC patients may be closely related to short-chain fatty acid and tryptophan metabolism.Conclusion:Patients with FC of different colonic transit dynamics exhibit distinct gut mi-crobiota characteristics.The characteristic dysbiosis of gut microbiota in FC patients with different colonic transit dynamics may be an important factor in the colonic motility disorders observed in FC.
万方数据
维普
