Effect of Zhizhu Kuanzhong capsule on the improvement of micro-inflammation in the duodenum of rats with functional dyspepsia
Objective:To investigate the interventional effect of Zhizhu Kuanzhong capsule(ZZKZ)on duode-nal microinflammation in rats with functional dyspepsia(FD).Methods:Thirty-six SD rats were randomly divided into the normal group and the model group,and the FD rats were established by the composite modeling method(iodoacetamide gavage and small platform standing).The successfully modeled FD rats were randomly divided in-to the model group,mosapride group and low,medium and high dose ZZKZ group.After 2 weeks of drug admin-istration,the general condition of rats in each group was observed,the body mass and gastric emptying rate of rats in each group were detected.The levels of IL-1β and IL-18 in the serum of each group were determined using an enzyme-linked immunosorbent assay,and the expression levels of NF-κB proteins were detected by Western blotting.Results:Compared with the model group,rats in ZZKZ group and the mosapride group showed improve-ments after drug intervention.Their fur became gradually clean and shiny,spontaneous activity frequency in-creased,response speed quickened,stool returned to normal,and body weight showed an upward trend(P＜0.05).Hematoxylin-eosin staining revealed that the gastric tissue structure of rats in all groups remained intact,with cells arranged neatly.Compared with the model group,rats in ZZKZ group and the mosapride group had du-odenal villi arranged neatly,with reduced infiltration of inflammatory cells and clearer intercellular spaces in hema-toxylin-eosin stained duodenal tissue.Compared with the model group,the serum levels of IL-18 and IL-1β in the medium and high dose ZZKZ group and mosapride group were reduced(P＜0.05).The expression of NF-Κb pro-tein in duodenal tissues was reduced in both the medium and high dose ZZKZ group and the mosapride group com-pared with the model group(P＜0.05).Conclusion:Zhizhu Kuanzhong capsule can effectively inhibit duodenal mi-croinflammation.
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