MitoTEMPO Alleviates High Glucose-induced Renal Tubular Epithelial Cell Injury by Regulating PGC-1 α/TFAM Signaling Pathway
Objective:To explore the mechanism of MitoTEMPO in alleviating high glucose-induced human tubular epithe-lial cell injury.Methods:Human proximal tubular epithelial cells were divided into control,high glucose(30 mmol/L glucose)and experimental(10 μmol/L MitoTEMPO +30 mmol/L glucose),Cell survival rate was measured by CCK-8,apoptosis rate was deter-mined by flow cytometry,expression levels of malondialdehyde(MDA),superoxide dismutase(SOD)and glutathione(GSH)were determined by ELISA,and reactive oxygen species(ROS)was measured by chemical fluorescence method.The expression levels of PGC-1 αand TFAM mRNA in each group were determined by qRT-PCR.The expression levels of PGC-1 αand TFAM in each group were determined by Westernblot.Results:Compared with the control group,the cells had decreased activity,increased apopto-sis rate(P<0.05),MDA and ROS expression levels(P<0.05),SOD and GSH expression levels(P<0.05),and PGC-1 α and TFAM protein and mRNA expression levels(P<0.05).Compared with the high glucose group,the cell survival rate was increased,the apoptosis rate was decreased(P<0.05),MDA and ROS expression levels were significantly decreased(P<0.05),SOD and GSH expression levels were significantly increased(P<0.05),and PGC-1 αand TFAM protein and mRNA levels were increased(P<0.05).Conclusion:MitoTEMPO can inhibit the high-glucose-induced oxidative stress and damage in renal tubular epithelial cells,and its mechanism may be related to the regulation of the PGC-1 α/TFAM signaling pathway.
Renal tubular epithelial cellMitoTEMPOPGC-1α/TFAM signal pathwayDiabetic kidney disease
NETL
NSTL
万方数据
