Bushen Huoxue Formula Alleviated Inflammation and Renal Fibrosis in a Unilateral Ureteral Obstruction Rat Model by Inhibiting JAK/Stat3 Pathway
Objective:This study is to observe the therapeutic effect of Bushen Huoxue Formula(BSHXF)on Inflammation and Renal Fibrosis in rats with Unilateral ureteral obstruction(UUO),thus revealing the mechanism of its anti-renal fibrosis and in-flammation.Methods:32 8-week-old female SD rats were randomly divided into Sham group(sham surgery group),UUO group(unilateral ureteral obstruction group),BSHXF-L group(low-dose group of BSHXF),and BSHXF-H group(high-dose group of BSHXF)based on a random number table.Continuous gastric lavage intervention was performed for 21 days from the second day af-ter modeling.The BSHXF-L and BSHXF-H groups were given corresponding doses of BSHXF,while the Sham and UUO groups were given physiological saline of the same volume as the BSHXF group.Results:After the intervention,24-hour urine output was recorded;a fully automated biochemical analyzer was used to detect blood creatinine,urea nitrogen,urinary protein,urinary creati-nine,and renal function index;HE and Masson staining were used to detect the degree of pathological damage and fibrosis;Analysis of TGF-β and TNF-α in each group by immunohistochemistry staining;Western blotting detection of TGF-β1、α-SMA、JAK、STAT3、p-STAT3、TNF-α、The protein expression of Caspase-3 and Bax in each group.Conclusion:Compared with the UUO group,the BSHXF-L and BSHXF-H groups significantly improve renal function of SD with UUO,reduced the expression level of TGF-β1 and α-SMA protein in rat kidney tissue and inhibited the expression of JAK,STAT3,and p-STAT3,reduced the infiltra-tion of inflammatory cells and the proliferation of fibrous tissue and collagen,significantly reduced the renal index of rats,and the oth-er inflammatory indicators of TNF-α et al in rats'kidneys.Conclusion:BSHXF can effectively improve renal function,alleviate in-flammation and renal fibrosis in rats with unilateral ureteral ligation by inhibiting of JAK/STAT3 pathway signaling.
Bushen huoxue formulaRenal fibrosisJAK/STAT3A rat model of unilateral ureteral obstruction
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