Comparison of Different Combinations of Huayu Tongluo Decoction on Glomerular Charge Barrier in Diabetic Rats
Objective:To explore the mechanism of different combinations of huayu tongluo decoction on proteinuria in dia-betic kidney disease.Methods:10 of 75 SD rats were randomly selected as control group,and the rest rats were fed with high-sugar and high-fat diet combined with intraperitoneal injection of streptozotocin to establish diabetic disease model.The diabetic rats were randomly divided into 15 rats in model group(M group),15 rats in huayu tongluo decoction group(Q group),15 rats in huayu de-coction group(H group)and 15 rats in tongluo decoction group(T group).The Chinese medicine group was given huayu tongluo de-coction by gavage,and the control group and model group were given the same amount of drinking water by gavage once a day for a to-tal of 16 weeks.Body weight,water intake,food intake,urine volume,random blood glucose,and 24-hour urine total protein(24 h UTP)were measured at the end of 16 weeks;and heparanase(HPA)、Glomerular epithelial protein 1(GLEPP1)、podocalyxin(PCX)in kidney and PCX in urinary were detected.Results:Compared with M group,24 h UTP decreased in each treatment group(P<0.05);compared with Q group,it increased in both H group and T group(P<0.05).Compared with M group,the expression of HPA protein decreased in Q group and T group(P<0.05);the expressions of GLEPP1 and PCX protein increased in Q group(P<0.05);the urinary PCX content decreased in each treatment group(P<0.05),and the content in Q group and T group was lower than in H group(P<0.05).Compared with M group,the HPA mRNA expression decreased in Q group and T group(P<0.05);the expression of PCX mRNA increased in Q group(P<0.05).Conclusion:The mechanism of huayu tongluo decoction to reduce proteinuria in diabetic kidney disease may be related to maintaining the integrity of the glomerular charge barrier.Different combinations of huayu tongluo decoction have different degrees of therapeutic effect,and the effect of the whole decoction is better than huayu decoction and tongluo decoction used alone.
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NSTL
万方数据
维普
