To Investigate the Protective Effect of Jiawei Xiaozheng Decoction on Renal Fibrosis in Chronic Renal Failure Rats and Its Effect on TGF-β1/Smad3 Signaling Pathway in Renal Tubular Epithelial Cells
Objective:To investigate the protective effect of Jiawei Xiaozheng Decoction(Jiawei Xiaozheng Decoction)on renal fibrosis in chronic renal failure(CRF)rats and its effect on TGF-β1/Smad3 signaling pathway in renal tubular epithelial cells.Methods:We observed the effects of Jiawei Xiaozheng Decoction on serum creatinine,urea nitrogen,24-hour urine protein,renal pathology and the expression levels of FN1,Col-Ⅰ,and Col-Ⅲ in chronic renal failure rats.To observe the effect of Jiawei Xi-aozheng recipe on the expression of TGF-β1,Smad3,LC3,Beclin1 and P62 in renal tubular cell injury induced by TGF-β1.Re-sults:Compared with the model group,the Scr level in the high-dose Jiawei Xiaozheng Decoction group and the 24 h urinary protein level in the Jiawei Xiaozheng group and valsartan group were significantly decreased(P＜0.05,P＜0.01);Real-time fluorescence quantitative PCR showed that the relative mRNA expression of FN1 and Col-Ⅰin the high-dose Jiawei Xiaozheng Decoction group,the relative mRNA expression of Col-Ⅲ in the medium-dose Jiawei Xiaozheng Decoction group and valsartan group,and the rela-tive mrna expression of TGF-β1 in the high-dose group and valsartan group The relative expression of Smad3 mRNA and the relative expression of Smad3 mRNA in the high,middle and low dose groups and valsartan group were significantly lower than those in the model group(P＜0.05,P＜0.01);Western The protein expressions of FN,Col-Ⅰ,Col-Ⅲand Beclin1 in the high-,medium-and high-dose Jiawei Xiaozheng Decoction groups and valsartan groups were significantly lower than those in the model group(P＜0.05,P＜0.01);Masson staining showed that the degree of renal interstitial fibrosis in the high-dose Jiawei Xiaozheng decoc-tion group was significantly reduced(P＜0.01).In addition,the 48 h/72 h cell survival rate was significantly increased(P＜0.01).Conclusion:Jiawei Xiaozheng Decoction can effectively improve the cell survival rate and the degree of renal interstitial fibro-sis,which may be achieved by regulating the level of autophagy through the TGF-β1/Smad3 pathway and down-regulating the lev-els of FN1,Col-Ⅰ,and Col-Ⅲ.
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