Effect of Yellow Sunflower Extract Powder on Fibrosis of Human Renal Tubular Epithelial Cells Induced by High Glucose
Objective:To investigate the effect of Huangkui extract powder on the fibrosis of human renal tubular epithelial cells(HK2)induced by high glucose,and to explore the possible mechanism of improving renal fibrosis.Methods:CCK8 was used to detect cell proliferation after 24 h,48 h and 72 h intervention with different concentrations of Huangkui extract powder,and the op-timal intervention concentration was 1 g/L and 48 h.Grouping:Normal group(glucose 5.5 mmol/L),mannitol group(glucose 5.5 mmol/L+mannitol 24.5 mmol/L),high glucose group(glucose 30 mmol/L)and yellow sunflower group(glucose 30 mmol/L+yellow sunflower extract powder 1 g/L),intervention for 48 hours,and mannitol group(glucose 5.5 mmol/L+mannitol 24.5 mmol/L).qPCR and Western Blot were used to detect the expressions of fibrosis-related proteins α-SMA,FN,TGF-β1,mitochondrial fis-sion protein Drp1 and mitochondrial fusion protein mfn2,and transmission electron microscopy was used to observe mitochondrial mor-phology.Results:(1)Compared with the normal group,the mRNA and protein expressions of α-SMA,FN,TGF-β1 and Drp1 in the high glucose group were significantly increased(P<0.001);The mRNA and protein expressions of Mfn2 were significantly de-creased(P<0.001).(2)Compared with the normal group,there was no difference in the indexes of the mannitol group(P>0.05),that is,high osmolality had no effect on HK2.(3)Compared with the high glucose group,the mRNA and protein expressions of α-SMA,FN,TGF-β1 and Drp1 in the Huangkui group were significantly decreased(P<0.01),and the mRNA and protein ex-pressions of Mfn2 were significantly increased(P<0.01).(4)Transmission electron microscopy:the number of mitochondria in the normal group and the mannitol group was large,and the morphology was normal.Swollen mitochondria,more mitochondrial frag-ments,mitochondrial matrix and mitochondrial cristae disruption were observed in the high glucose group.Huangkui extract powder can reduce mitochondrial damage after intervention.Conclusion:Huangkui extract powder can reduce the production of α-SMA,FN and TGF-β1,and inhibit HK2 fibrosis induced by high glucose.At the same time,it can promote the expression of mitochondrial fu-sion protein Mfn2 and reduce the expression of mitochondrial fission protein Drp1,thereby reversing renal fibrosis and delaying the progression of diabetic nephropathy.
HK2Huangkui extract powderHigh glucoseFibrosisMitochondrial fission and fusion
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