Objective:To explore the effect of Baicalin on peritoneal fibrosis and angiogenesis in peritoneal dialysis rats by regulating hypoxia inducible factor-1 α/vascular endothelial growth factor(HIF-1 α/VEGF)signaling pathway.Methods:SD rats were grouped into blank group,model group,low-dose Baicalin group(25 mg/kg),high-dose Baicalin group(100 mg/kg),HIF-1α activator FG-4592 group(10 mg/kg),high-dose Baicalin+FG-4592 group(100 mg/kg Baicalin+10 mg/kg FG-4592),with 10 rats in each group.In addition to the blank group,4.25％glucose peritoneal dialysis solution was intraperitoneally injected into other groups to establish peritoneal dialysis rat models.The rats in each group were given corresponding drug treatment,once a day,for a total of 30 days.The blank group and the model group were given equal volumes of physiological saline.The 4-hour peritoneal balance test was applied to evaluate peritoneal function;HE and Masson staining were applied to observe the pathologi-cal damage,thickness,and degree of fibrosis in peritoneal tissue;the morphology of peritoneal angiogenesis was observed;immuno-histochemistry was applied to detect the expression of α-smooth muscle actin(α-SMA),VEGF and epithelial cadherin in perito-neal tissue;real-time fluorescence quantitative-PCR(qRT-PCR)and Western Blot were applied to measure the expression levels of HIF-1α and VEGF in peritoneal tissue.Results:Compared with the blank group,the ultrafiltration volume and the expression lev-el of E-cadherin in the model group were obviously reduced,the peritoneal thickness,glucose transport capacity,capillary density,level of α-SMA,and expression of HIF-1 α and VEGF were obviously increased,there was a large amount of inflammatory cell in-filtration and blue stained collagen deposition in the peritoneal tissue of rats(P＜0.05);compared with the model group,the ultrafil-tration volume and E-cadherin expression level of rats in the low-dose Baicalin group and the high-dose Baicalin group increased obviously,the ultrafiltration volume and the expression level of E-cadherin in the low-dose Baicalin group and the high-dose Ba-icalin group were obviously increased,the peritoneal thickness,glucose transport capacity,capillary density,level of α-SMA,and expression of HIF-1 α and VEGF were obviously reduced,the pathological damage and fibrosis of rat peritoneal tissue improved(P＜0.05),the change trend of corresponding indicators in the FG-4592 group was opposite to the above(P＜0.05);FG-4592 atten-uated the effect of high-dose Baicalin in inhibiting peritoneal fibrosis and angiogenesis in peritoneal dialysis rats.Conclusion:Baica-lin inhibits peritoneal fibrosis and angiogenesis in peritoneal dialysis rats by inhibiting HIF-1 α/VEGF signaling pathway.
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