太灵丹含药血清调控PI3K/AKT/GSK3β通路减轻高糖诱导的足细胞转分化的机制研究
Tailing Pills-containing Serum Inhibits Podocyte Hyperglycemia-induced Epithelial Mesenchymal Transition Via PI3K-AKT-GSK3β Pathway
李敏州 1王权 1刘岩 1党林林 1张慧艳 2牛洋 2邓斌 2刘宇幸2
作者信息
- 1. 内蒙古自治区中医医院内分泌科(呼和浩特 010010)
- 2. 内蒙古医科大学中医学院(呼和浩特 010110)
- 折叠
摘要
目的:观察太灵丹(TLD)含药血清对高糖诱导的足细胞转分化及PI3K/AKT/GSK3β通路的影响及分子机制.方法:将小鼠肾脏足细胞MPC5分为空白对照组、高糖刺激组(模型组)、太灵丹血清组(高、中、低浓度).CCK-8检测足细胞的活力,流式细胞检测足细胞的凋亡,免疫组化检测desmin、FSP-1的蛋白表达,Western blot和QPCR法检测足细胞podocin、CD2-AP、FSP-1、PI3K、Akt、p-Akt、GSK3β蛋白及mRNA表达.结果:与正常组相比,模型组足细胞活力下降(P＜0.01),凋亡增加(P＜0.01),免疫组化结果显示,desmin及FSP-1的蛋白表达较正常组升高(P＜0.05),podocin、CD2-AP、PI3K、Akt、p-Akt的蛋白及mRNA表达明显降低(P＜0.05),而desmin、FSP-1、GSK3β的蛋白及mRNA表达明显升高(P＜0.05).与模型组相比,太灵丹含药血清各组足细胞活力显著升高(P＜0.01),凋亡减少(P＜0.01),podocin、CD2-AP、PI3K、Akt、p-Akt的蛋白及mRNA表达均明显上调(P＜0.05),desmin、FSP-1、GSK3β的蛋白及mRNA表达均明显下调(P＜0.05).结论:太灵丹含药血清更能够减轻高糖诱导的足细胞转分化,其部分作用机制是通过调控PI3K/AKT/GSK3β通路实现的.
Abstract
Objective:To observe the effects of drug-containing serum of Tailing Pills on glomerular podocyte epithelial-mesenchymal transition(EMT)and PI3 K/AKT/GSK3 β pathway under high glucose conditions,and to explore the protection mecha-nism for podocyte injury.Methods:Cultured differentiated mouse podocytes were treated with a drug-containing serum of Tailing Pills.After treating 48 hours,podocytes viability was determined by CCK-8 assay,the markers of podocyte EMT and the molecules related to PI3K/AKT/GSK3β pathway were analyzed by real-time PCR and Western Blot.Results:Compared with normal group,the model group's gene and protein expressions of Podocin,CD2-AP,PI3K,Akt and p-Akt of podocytes induced by hyperglycemia in model group were decreased(P＜0.05).The gene and protein expressions of Desmin,FSP-1 and GSK3β were increased(P＜0.05).The podocyte viability was decreased(P＜0.01)and apoptosis was increased(P＜0.01).Compared with model group,the Tailing Pills group's gene and protein expressions of Podocin,CD2-AP,PI3K,Akt and P-Akt were significantly increased(P＜0.05),and the gene and protein expressions of Desmin,FSP-1 and GSK3β were decreased(P＜0.05).The podocyte viability was increased(P＜0.01)and apoptosis was decreased(P＜0.01).Conclusion:Drug-containing serum of Tailing Pills can inhibit hy-perglycemia-induced podocyte EMT,and the underlying mechanism is partially through regulating PI3K/AKT/GSK3β pathway.
关键词
太灵丹/足细胞/转分化/PI3K/AKT/GSK3β通路Key words
Tailing pills/Podocyte/Epithelial-mesenchymal transition/PI3K/AKT/GSK3β signaling pathway引用本文复制引用
基金项目
2022年度内蒙古自治区卫生健康科技计划项目(202201044)
内蒙古医学科学院公立医院科研联合基金科技项目(2023GLLH0118)
出版年
2024
NETL
NSTL
万方数据