Objective:To investigate the application value of high-frequency ultrasound in the diagnosis of the anterior talofibular ligament(ATFL)injury and avulsion fracture caused by acute ankle sprain.Methods:83 patients with ATFL injury and avulsion fracture were included retrospectively.ATFL injury grading was made according the ultrasound imaging,and blood flow signals on the ATFL and features of avulsion fracture were analyzed.Results:Among 83 patients,blood flow singles were detected on the injured ligament in 57 cases(68.7%),and it was more likely to detect blood flow singles in type Ⅱ injuries than in type Ⅰ injuries(P<0.001).There was a positive correlation between blood flow grading and the degree of ligament injuries(rs=0.333,P<0.05).Avulsion fracture was more common at the end of fibular,and single avulsion bone fragment was common.Among 39 patients underwent surgical treatment,the intraoperative findings of 37 cases(94.9%)were consistent with ultrasound findings.Among 44 patients with conservative treatment and MRI examination,the MRI findings of 39 cases(88.6%)were consistent with the ultrasound findings.Conclusion:High-frequency ultrasound can be used as the first choice for clinical diagnosis of ATFL injury complicated with avulsion fracture.