Differential Expression Profile of miRs in Bone Tissue of Steroid-Induced Osteonecrosis of the Femoral Head Based on High-Throughput Sequencing Technology
Objective:To investigate the differential expression profile of micrornas(miRs)in bone tissue of steroid-induced avascular necrosis of the femoral head(SANFH).Methods:31 patients who underwent total hip arthroplasty due to SANFH and tibia fractures from June 2019 to January 2023 were selected and recorded as SANFH group and control group respectively.The Illumina high-throughput sequencing technology was used to sequence the bone tissues of SANFH patients and femoral neck fracture patients,and the miRs differences between groups were screened.Bioinformatics was used to analyze the difference of gene expression profiles between the two populations.Results:Through Illumina high pass sequencing,1 668 known miRNAs were compared and 107 new miRNAs were predicted.Compared with the control group,there were 76 differentially expressed miRNAs(log2(FoldChange)＞1.5,P＜0.05)in bone tissue of patients with SANFH,including 40 up-regulated miRNAs and 36 down-regulated miRNAs.Target gene prediction was conducted for 61 differentially expressed miRs.Go analysis showed that the target gene functions regulated by the two groups of differentially expressed miRs mainly include:cell metabolic process,intracellular signal transduction,mesenchymal cell proliferation,cell killing,receptor binding,protein activity,enzyme regulation activity,anatomical structure development,etc.The enrichment analysis of Kyoto encyclopedia of genes and genomes(KEGG)pathway showed that the signal pathways regulated by the two groups of differentially expressed miRs mainly included:peroxisome proliferator activated receptor γ(PPARγ)signal pathway,Janus kinase(JAK)/signal transducer activator of transcription(STAT)signal pathway,transforming growth factor-β1(TGF-β1)/Smad3 pathway,phosphatidylinositol 3 kinase(P13K)/serine threonine protein kinase(Akt)signal pathway,T cell receptor signal path-way,vascular endothelial growth factor(VEGF)signal pathway,ubiquitin proteasome pathway,etc.Conclusion:There are differentially expressed miRs in SANFH bone tissue,which can act on PPARy,JAK/STAT,TGF-β1/Smad3,P13K/Akt and other signal pathways participate in the occurrence and development of SANFH.
high throughput sequencing technologysteroid induced avascular necrosis of femoral headbone tissuemicro ribonucleic acidbioinformatics
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万方数据
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