Sonic Hedgehog Factor-Loaded Sodium Alginate Hydrogel on the Repair of Rat Knee Articular Cartilage Defects
Objective:To investigate the effect of sonic hedgehog factor(Shh)-loaded sodium alginate hydrogel on the repair of the rat knee articular cartilage defects.Methods:Human bone marrow mesenchymal stem cells(BMSCs)were cultured.Shh-loaded sodium alginate hydrogel was prepared.A randomized controlled animal observation experiment was conduc-ted.48 healthy SPF male SD rats aged 6 weeks were selected to establish knee cartilage defect models.They were random-ly divided into three groups:injury group,observation group,and control group,with 16 rats in each group.The injury group did not receive any treatment,the control group was implanted with 25 μL of Shh-free sodium alginate hydrogel and 25 μL of BMSCs suspension,and the observation group was implanted with 25 μL of Shh-loaded sodium alginate hydrogel and 25 μL of BMSCs suspension.After 12 weeks,the rats in each group were euthanized.and the effect of cartilage defect and repair were observed.International cartilage repair society(ICRS)scores were assigned,and histological changes in cartilage tissue were evaluated using hematoxylin and eosin(HE)staining and Safranin O staining.Immunohistoche-mistry was performed to detect the expression of type Ⅱ collagen in each group.The qRT-PCR was used to measure the mRNA expression levels of Sox9 and glycosaminoglycan(GAG)in each group.Results:Compared with the injury group,the ICRS scores of the control group and observation group rats increased,the cartilage tissue injury of knee joint was significantly improved,and the cartilage thickness was significantly increased.The expression levels of type Ⅱ collagen,Sox9 mRNA,and GAG mRNA were up-regulated in both groups(P＜0.05).Compared with the control group,the ICRS scores of the observation group rats increased,the carti-lage tissue injury of knee joint was significantly improved,and the cartilage thickness was significantly increased.The expression levels of type Ⅱ collagen,Sox9 mRNA,and GAG mRNA were up-regulated in the observation group(P＜0.05).Conclusion:Shh-loaded sodium alginate hydrogel exhibits excellent reparative effects on knee articular cartilage defects.
sonic hedgehog factorsodium alginate hydrogelknee articular cartilage defecttype Ⅱ collagen
万方数据
维普
