乌头汤对骨关节炎软骨细胞中基质金属蛋白酶13和肿瘤坏死因子-α表达的影响机制研究

扫码查看

原文链接

万方数据
维普

中文摘要：目的:探讨乌头汤对骨关节炎(OA)软骨细胞中基质金属蛋白酶13(MMP-13)和肿瘤坏死因子-α(TNF-α)表达的影响，以及其对沉默信息转录调控因子1(Silent Information Regulator 1，SIRT1)/转录因子叉头框蛋白O1(Forkhead Box Transcription Factor O1，FOXO1)信号的调控机制。方法:以脂多糖模拟炎性微环境构建骨关节炎软骨细胞模型;以SIRT1信号特异性抑制剂EX527做功能挽救实验，细胞实验分为正常对照组、脂多糖组、乌头汤低剂量组、乌头汤高剂量组、抑制剂组;钙黄绿素-AM(Calcein-AM)/碘化丙啶(Propidium Iodide，PI)染色检测各组细胞的生长活性;流式细胞仪检测各组细胞的凋亡率，免疫荧光检测各组细胞中TNF-α和 MMP-13的表达;免疫组化检测各组细胞中蛋白聚糖(Aggrecan)和Ⅱ型胶原(Collagen Ⅱ)的表达;Western Blot法检测细胞和软骨组织中SIRT1、乙酰化叉头转录因子 1(Ac-FOXO1)、B 淋巴细胞瘤-2(B Cell Lymphoma-2，Bcl-2)和 Bcl-2 相关 X 蛋白(Bcl2-associated X Protein，Bax)的表达。结果:在骨关节炎细胞模型中，细胞的生长活性明显降低，凋亡率明显升高，TNF-α 和 MMP-13 以及 Ac-FOXO1 和 Bax 的表达明显升高，Aggrecan 和 Collagen Ⅱ、SIRT1和Bcl-2的表达明显降低，乌头汤低、高剂量能明显阻滞这些参数的恶化，发挥保护软骨细胞的作用，而EX527可部分逆转乌头汤的保护作用，差异均有统计学意义(P＜0。05)。结论:乌头汤能明显下调TNF-α和MMP-13的表达，抑制骨关节炎软骨细胞的凋亡，这可能与激活SIRT1信号、抑制FOXO1的乙酰化有关。

外文标题：Mechanism Study of Wu-tou Decoction on the Expression of Matrix Metalloproteinase-13 and Tumor Necrosis Factor-α in Osteoarthritis Chondrocytes

外文摘要：Objective:To investigate the effect of Wu-tou decoction on the expressions of matrix metalloproteinase 13(MMP-13)and tumor necrosis factor-α(TNF-α)in osteoarthritis(OA)chondrocytes cell,and its regulatory mechanism on silent information regulator 1(SIRT1)/forkhead box transcription factor O1(FOXO1)signaling pathway.Methods:Lipopolysaccharide was used to simulate inflammatory microenvironment to construct osteoarthritis chondrocyte model.EX527,SIRT1 signal specific inhibitor,was used to perform functional rescue experiment.Cell experiments were divided into normal control group,lipopolysaccharide group,low dose group of Wu-tou decoction,high dose group of Wu-tou decoction and inhibitor group.Calcein AM/propidium iodide(PI)staining was used to detect the growth activity of cells in each group.Flow cytometry was used to detect the apoptosis rate.Immunofluorescence was used to detect the expression of TNF-α and MMP-13.Immunohistochemistry was used to detect the expression of Aggrecan and Collagen Ⅱ.Western Blot was used to detect the expression of SIRT1,acetylated FOXO1(Ac-FOXO1),B cell lymphoma-2(Bcl-2)and Bcl-2 associated X protein(Bax)in cells.Results:In osteoarthritis cells,the growth activity of cells decreased significantly,the apoptosis rate increased significantly,the expressions of TNF-α and MMP-13,Ac-FOXO1 and Bax were increased signifi-cantly,and the expressions of Aggrecan and Collagen Ⅱ,SIRT1 and Bcl-2 were decreased significantly.Low and high dose of Wu-tou decoction could significantly block the deterioration of these parameters and exert a protective effect of chondro-cytes,while EX527 could partially reverse the protective effect of Wu-tou decoction.The differences were statistically sig-nificant(P＜0.05).Conclusion:Wu-tou decoction could significantly down-regulate the expression of TNF-α and MMP-13 and inhibit the apoptosis of chondrocytes in osteoarthritis,which may be related to the activation of SIRT1 signal and inhi-bition of FOXO1 acetylation.

外文关键词：

Wu-tou decoctionosteoarthritismatrix metalloproteinase 13tumor necrosis factor-αsilent information reg-ulator 1/forkhead box transcription factor O1 signaling pathway

作者：

刘李梅、彭伟、吴明权、凃禾

展开 >

作者单位：

四川省骨科医院(成都,610041)

关键词：

乌头汤骨关节炎基质金属蛋白酶13 肿瘤坏死因子-α 沉默信息转录调控因子1/转录因子叉头框蛋白O1信号通路

基金：

四川省科技创新苗子工程资助项目

项目编号：

2020099

出版年：

2024

DOI：

10.20085/j.cnki.issn1005-0205.240203

中国中医骨伤科杂志

中华中医药学会,湖北省中医药研究院

中国中医骨伤科杂志

CSTPCD

影响因子：0.732

ISSN：1005-0205

年,卷(期)：2024.32(2)

参考文献量22