Effect of Huoxue Zhitong Decoction Combined with Fat Filling on the Injury of Fibial Epiphyseal in Fats
Objective:To investigate the effect of Huoxue Zhitong decoction combined with fat filling on the type Ⅲ and Ⅳepiphyseal injury of distal tibia in rats.Methods:36 rats were randomly divided into control group,model group,adipose group and adipose Chinese medicine group.The establishment of injury model,the healing process of epiphyseal injury,bone bridge formation and the expression of vascular endothelial growth factor(VEGF),bone morphogenetic protein-2(BMP2)and interleukin-1β(IL-1β)were observed by alcin blue staining and immunofluorescence staining.Results:Com-pared with the control group,alcin blue staining showed that the epiphyseal injury was obvious in the model group,and bone bridge was formed.However,after the intervention of adipose and Huoxue Zhitong decoction,the epiphyseal injury was obviously improved and bone bridge disappeared,and the effect of adipose Chinese medicine group was more obvious.Immunofluorescence results indicated that compared with the control group,the expressions of VEGF,BMP2 and IL-1β were significantly increased in the model group,while the expressions of VEGF,BMP2 and IL-1β were decreased in the fat group and the fat Chinese medicine group,and the expressions of VEGF,BMP2 and IL-1β were more significantly decreased in the fat Chinese medicine group.Conclusion:The therapeutic efficacy of Huoxue Zhitong decoction combined with fat filling on the type Ⅲ and Ⅳ epiphyseal injury of distal tibia in rats is significantly better than simple fat interven-tion.
type Ⅲ and Ⅳ epiphyseal injury of distal tibia in ratsHuoxue Zhitong decoctionfat fillingbone bridgecartilage cells
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