Efficacy and Mechanism of Tetramethylpyrazine in Repair of Sciatic Nerve Injury in Rats
Objective:To explore the mechanism of tetramethylpyrazine(TMP)on the treatment of sciatic nerve injury(SNI)by regulating the basal membrane of nerve vessels.Methods:Sprague Dawley(SD)rats were randomly divided into control group,the model of sciatic nerve crush injury was made by means of hemostatic forceps.These rats were randomly divided into sham operation group(NC group),tetramethylpyrazine group(TMP group,200 mg/kg),and normal saline group(NS group,200 mg/kg).Sciatic nerve function index(SFI)and grid crawling experiments were used for behavioral evaluation.Hematoxylin-eosin(HE)staining was performed to observe its pathological structure and morphology.Immu-nohistochemistry was used to assess the expression level of Collagen Ⅳ and Laminin expression in sciatic nerve vascular basal membrane.Results:SFI results showed that there were significant differences between the two modeling groups and the NC group(P＜0.01).The TMP group had a significant increase in the SFI compared with the NS group(P＜0.01).The results of grid crawling experiments showed that there were significant differences between the two modeling groups and the NC group(P＜0.01).The miss times in TMP group was significantly lower than that in NS group(P＜0.01).The HE staining results of the TMP group showed irregular arrangement of some neuronal axons and Schwann cells,and more edema and rupture of cells compared with the NC group.The proliferation of glial cells and inflammatory cells was significantly increased in TMP group compared with the NC group.The results of immunohistochemistry showed that the expression of type Ⅳ collagen and laminin in the TMP group and NS group was distributed around the blood vessels,vas-cular endothelial cells,basal membrane and glial cells after SNI,and the expression in NS group was mainly distributed around blood vessels.The expression of type Ⅳ collagen and laminin in TMP group increased significantly compared with the NC group(P＜0.05,0.01),and the analysis results in NC group were negative.Conclusion:TMP can effectively im-prove the SFI of rats,enhance the proliferation of sciatic nerve vascular endothelial cells,reduce apoptosis,promote the expression of Collagen Ⅳ and Laminin in sciatic nerve microvascular basal membrane components,thereby promoting an-giogenesis and improving lower limb function in rats after SNI.
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