Experimental Study on the Efficacy of Yigu Decoction Regulating M-CSF Signal Axis on Osteoclast Differentiation in Osteoporosis Rats
Objective:To observe the efficacy of Yigu decoction on macrophage colony-stimulating factor(M-CSF)signal axis in bone tissue of osteoporosis rats,and to explore the mechanism of Yigu decoction in treating osteoporosis by interve-ning osteoclast differentiation.Methods:Forty 12-week-old female SD rats were randomly divided into sham operation group,model group,estrogen group and Yigu decoction group.Rats were killed and bilateral femurs were taken after 3 months of drug intervention.The bone mineral density were measured,the biomechanics were tested,the changes of bone trabeculae were observed by hematoxylin-eosin(HE)staining,and the expressions of M-CSF,c-Fms and Grb2 were detec-ted by Western Blot and qPCR.Results:Compared with the model group,the bone trabecular structure of estrogen group and Yigu decoction group was more complete,the continuity was better,the bone mineral density increased(P＜0.01),and the bending load,elastic modulus and rigidity increased significantly(P＜0.01).Compared with the sham operation group,the expression of c-Fms,Grb2 and PI3K protein in the femur of the model group increased(P＜0.05).Compared with the model group,the expression of c-Fms and PI3K pro-tein in the femur of the Yigu decoction group decreased sig-nificantly(P＜0.01,P＜0.05),and the expression of c-Fms protein in the estrogen group decreased significantly(P＜0.01).The expression of c-Fms mRNA decreased in sham operation group,estrogen group and Yigu decoction group(P＜0.01),PI3K mRNA decreased in estrogen group(P＜0.05),and Grb2 mRNA decreased in Yigu decoction group(P＜0.05).Conclusion:Yigu decoction can inhibit osteoclast differentiation,reduce bone resorption and improve bone strength in osteoporosis rats by regulating M-CSF signal axis.
Yigu decoctionosteoporosisM-CSF signal axisosteoclast
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