Study of Injection of Ozone Water into Joint Cavity in Promoting the Repair of Knee Cartilage Injury in Rats by Regulating Inflammation and Oxidative Stress
Objective:To study the optimal concentration,treatment frequency and treatment cycle of injection of ozone wa-ter into joint cavity to repair knee osteoarthritis(KOA)rat cartilage,and to explore the potential mechanism.Methods:210 SD rats with KOA cartilage injury model were prepared by sodium iodoacetate.The rats were treated with ozonated water(5,10,20 μg/mL)for 1,2 and 4 weeks according to the treatment frequency of once every 1,3 and 7 d respectively.After treatment,the diameter of the knee joint in each group were measured.Rats were killed and the articular cartilage surface of the rats were fully exposed the articular cartilage surface for cartilage surface gross score.Hematoxylin-eosin(HE)staining and modified Mankin's score were used to evaluate the repair of articular cartilage.Serum levels of inflammatory factors(IL-1β,TNF-α,IL-10)were determined by ELISA.The colorimetric cartridge was used to detect the level of oxida-tive stress(SOD,MDA).Results:When the treatment cycle was 1 week,the treatment with 20 μg/mL ozone water once every 3 d had the best effect on cartilage repair(P＜0.05).When the treatment cycle was 2 weeks and 4 weeks,the effect of cartilage repair once every 7 d was significantly better than once every 3 d(P＜0.05).ELISA results showed that injection of 20 μg/mL ozone water into articular cavity once every 7 d,after 2 weeks'treatment,the expression level of pro-inflammatory cytokine of IL-1β and TNF-α significantly reduced.The expression level of anti-inflammatory cytokine of IL-10 increased.The SOD activity was significantly increased,and the MDA level in KOA rats was decreased(P＜0.05).Conclusion:The 20 μg/mL ozone water is the optimal concentration,and once every 7 d for 2 weeks is the optimal treatment frequency and treatment cycle.Moreover,ozone water joint cavity injection may promote cartilage repair by regulating inflammation and oxidative stress,and is an effective candi-date for KOA therapy.
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