The Value of Serum miR-1297 and miR-4458 in the Diagnosis and Prognosis Evaluation of Osteosarcoma Patients
Objective:To investigate the expression levels of microRNA-1297(miR-1297)and microRNA-4458(miR-4458)in the serum of patients with osteosarcoma,and their value in diagnosis and prognosis evaluation.Methods:90 patients with osteosarcoma(osteosarcoma group)who underwent surgical treatment from January 2019 to January 2020 were collected as research subjects,and all patients were followed up for 3 years after surgery.According to survival status,patients were grouped into survival group(49 cases)and death group(41 cases).Another 90 healthy individuals who underwent physi-cal examination during the same period and matched the general information of osteosarcoma patients were regarded as the control group.The levels of serum miR-1297 and miR-4458 in each group were compared,multivariate Logistic regression was applied to analyze the influencing factors of prognosis in patients with osteosarcoma.Receiver operator characteristic(ROC)curve was applied to analyze the diagnostic value of serum miR-1297 and miR-4458 for osteosarcoma and their prognostic value.Results:The levels of miR-1297 and miR-4458 in the osteosarcoma group were reduced than those in control group.The differences were statistically significant(P＜0.05).The area under the curve(AUC)of the combined diagnosis of miR-1297 and miR-4458 for osteosarcoma was 0.863,with a sensitivity of 88.89％and a specificity of 65.56％,the combination of the two was superior to the individual diag-nosis of serum miR-1297 and miR-4458(Zcombination-miR-1297=3.043,Zcombination-miR-4458=3.826,P=0.002,＜0.001).The expression levels of miR-1297 and miR-4458 in serum of osteosarcoma patients with TNM stage ⅡB/Ⅲ and distant metastasis were lower than those of patients with TNM stage Ⅱ A and no distant metastasis,the differences were statistically significant(P＜0.05).The expression levels of serum miR-1297 and miR-4458 in the death group were lower than those in the survival group,the differences were statistically significant(P＜0.05).The combined prediction of serum miR-1297 and miR-4458 for the prognosis of osteosarcoma had an AUC of 0.951,a sensitivity of 97.56％,and a specificity of 83.67％,the combi-nation of the two was superior to their individual predictions(Zcombination-miR-1297=2.080,Zcombination-miR-4458=2.530,P=0.038,0.011).The results of multivariate Logistic regression analysis showed that serum miR-1297 and miR-4458 were protec-tive factors for the prognosis of osteosarcoma patients,while TNM staging and distant metastasis were risk factors(P＜0.05).Conclusion:Serum miR-1297 and miR-4458 are obviously down-regulated in patients with osteosarcoma,and the combination of the two has good reference value for diagnosing osteosarcoma and predicting prognosis.
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