Study on the Histocompatibility between iPSCs Derived Epidermal Stem Cells and Acellular Amniotic Membrane
Objective:To investigate the histocompatibility of epidermal stem cells derived from induced pluripotent stem cells(iPSCs)with human acellular amniotic membrane,and to explore the feasibility of using these cells to construct skin substitutes.Methods:By regulating the rheumatoid arthritis(RA),bone morphogenetic protein 4(BMP4),and epidermal growth factor(EGF)signaling pathways,iPSCs were directed to differentiate into epidermal stem cells.Subsequently,the cells were implanted into the human acellular amniotic membrane,and the biocompatibility of iPSCs derived epidermal stem cells with the human acellular amniotic membrane was detected using hematoxylin-eosin(HE)staining,Cell-Count-ing-Kit-8(CCK-8),and scanning electron microscopy.Results:iPSCs differentiated cells had similar morphology,pheno-type,and gene expression to epidermal stem cells.The cells grew in a tight and thin sheet like manner on the acellular am-niotic membrane,and had significant differences in proliferation compared to the control group.Conclusion:iPSCs derived epidermal stem cells have good biocompatibility with acellular amniotic membrane and are potential seed cells for skin sub-stitutes.
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