Effect of Jiedu Huayu Decoction Combined with Antibiotics on the Biofilm of Staphylococcus Epidermidis on Titanium Surface
Objective:To observe the inhibitory effect of Jiedu Huayu decoction combined with antibiotics on biofilm forma-tion and mature biofilm of staphylococcus epidermidis(SE)on titanium surface.Methods:Using drug-resistant staphylo-coccus epidermidis ATCC35984 as the study object,the minimum inhibitory concentration(MIC)and the fractional inhibi-tory concentration index(FICI)of the combination of Jiedu Huayu decoction,levofloxacin,cefotiam,clindamycin,gentamy-cin,and vancomycin were detected by using the microdilution method,respectively.Fractional inhibitory concentration index was used to screen out the drug combinations with strong inhibitory effects on SE biofilm.The effects of the combi-nation of drugs on the formation and maturation of SE biofilm on the titanium surface were observed by doubled dilution plate counting,crystal violet staining,scanning electron microscope(SEM)and confocal laser scanning microscope(CLSM).Results:The combination of Jiedu Huayu decoction with levofloxacin was a drug combination with strong inhibi-tory effect on SE biofilm.Secondly,both qualitative and quantitative analyses showed that the combination of Jiedu Huayu decoction and levofloxacin could inhibit the formation of SE biofilm on the titanium surface as well as destroy the SE mature biofilm.Conclusion:The combination of Jiedu Huayu decoction and levofloxacin can inhibit the formation of SE biofilm on titanium surface,and the strongest inhibitory effect was found in the early stage.Secondly,the combination of the two drugs can destroy SE mature biofilm,and to some extent,it can also kill the bacteria in the membrane.
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