Experimental Study on Photodynamic Antibacterial Therapy Combined with Gentamicin Bone Cement on the Treatment of Staphylococcus Aureus Osteomyelitis
Objective:To investigate the efficacy of photodynamic antibacterial therapy combined with gentamicin bone ce-ment on the treatment of staphylococcus aureus osteomyelitis through rat experiments.Methods:A SD rat model of femo-ral staphylococcus aureus osteomyelitis was constructed using surgery.The rats were divided into 4 groups:sham surgery group(blank group),osteomyelitis group(model group),gentamicin bone cement group(control group),and photody-namic antibacterial therapy combined with gentamicin bone cement group(observation group).Bone tissue was taken for bacterial isolation,culture,and identification after 4 weeks of modeling.The modeling was successful.The blank group on-ly underwent surgery to open the tissue at the same time.The model group did not undergo any treatment after win-dow opening and debridement.Gentamicin bone cement was injected into the bone marrow cavity of the infected femur in the control group after fenestration and debridement.Gentamicin bone cement was injected into the infected femoral bone marrow cavity and combined with photodynamic antibacterial therapy for treatment after fenestration and debridement in the observation group.X-ray observation of postoperative bone changes was performed at 4 and 12 weeks after surgery,and a modified Norden score was performed.Bone tissue and muscle tissue were taken for Hematoxylin-eosin(HE)stai-ning to observe their pathological changes and bacterial culture identification at 4 weeks after surgery.Results:The identifi-cation and modeling were successful 4 weeks after modeling.The X-ray results showed significant bone destruction accom-panied by dead bone formation at the surgical site in the model group after 12 weeks of treatment.It was observed that the periosteal connection at the surgical site was observed in the control group and observation group,and the observation group had better healing.The improved Norden score showed that the scores of the control group and the observation group decreased significantly at 4 and 12 weeks of treatment,indicating an improvement in osteomyelitis.The score of the model group did not decrease,and there was a statistical difference in the score compared to the observation group(P＜0.05).HE detection of muscle and bone tissue at the surgical site at 4 weeks showed that in the model group,a large amount of inflammatory infiltration was observed in bone tissue,and a large amount of collagen proliferation and decreased muscle fibers were observed in muscle tissue.The control group showed reduced inflammatory infiltration,increased muscle fibers,and decreased collagen ratio.The observation group had better inflammatory infiltration than the control group,with more and more complete muscle fiber recovery,and a lower collagen ratio than the control group,indicating that the observation group had better inhibition of bone marrow inflammation.The bacterial culture results showed that the model group had significant bac-terial proliferation,while the observation group and control group had less bacterial proliferation,and the observation group had better antibacterial efficacy compared to the control group(P＜0.05).Conclusion:Photodynamic antibacterial therapy can inhibit the proliferation of staphylococcus aureus,and combined with gentamicin bone cement has a better inhibitory efficacy on inflamma-tory infiltration of bone marrow and can promote muscle tissue recovery.
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