Role of NLRP3 Inflammatory Vesicle Activation-Mediated Cell Pyroptosis in Acute Soft Tissue Injury and the Antagonistic Efficacy of Erhuangxinshang Analgesic Ointment
Objective:To investigate the inhibitory efficacy of Erhuangxinshang analgesic ointment on NOD-like receptor family,Pyrin domain containing 3(NLRP3)inflammatory vesicle activation-mediated inflammatory response and pyropto-sis on the treatment of acute soft tissue injury.Methods:SD rats were randomly divided into normal group,model group,ointment treatment group and Caspase-1 inhibitor treatment group,and acute soft tissue injury model was established in addition to normal group.The rats in each group were scored for soft tissue injury during the treatment.Rat soft tissues were stained with Hematoxylin-eosin(HE)to observe pathological changes.Rat soft tissue cells were stained with TUNEL staining to observe pyroptosis.The ultrastructure of injured tissues was observed by scanning electron micro-scope(SEM).Western Blot was used to detect the protein expression level of cell death related factors.The content of IL-1β and IL-18 in rat serum was detected by ELISA.Results:In comparison with the model group,the soft tissue injury scores decreased day by day in rats treated with Erhuangxinshang analgesic ointment or Caspase-1 inhibitor.The patholog-ical changes of muscle tissue were improved.The pyroptosis rate of soft tissue cells was significantly reduced.The arrangement of the muscle fibres in the soft tissue tends to dissolve from disorganised to neat and clear.There was a sig-nificant decrease in the expression levels of NLRP3 and GSDMD-N proteins,and the expression of Caspase-1 and Cleaved Caspase-1 protein decreased to some extent,signifi-cant reduction in serum levels of IL-1β and IL-18.Conclusion:Erhuangxinshang analgesic ointment can inhibit the inflam-matory reaction and pyroptosis mediated by the activation of NLRP3 inflammatory bodies,thus achieving the antagonistic efficacy on acute soft tissue injury.
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