Experimental Study of Xishu Decoction on Knee Osteoarthritis with Syndrome of Yang Deficiency and Cold Coagulation
Objective:To evaluate the effect of Xishu decoction on knee osteoarthritis(KOA)with syndrome of Yang defi-ciency and cold coagulation(KOA-YDCC)in rabbits and investigate its mechanisms.Methods:The KOA rabbits were established by papain injection,and the syndrome of Yang deficiency and cold coagulation was induced by peppermint oil gavage combined with a wet and cold environment created by climate box.The experimental rabbits were divided at ran-dom into 5 groups(12 cases in each group):model,positive control(celecoxib 100 mg/kg),Xishu decoction high and low dose(16 and 8 g/kg,respectively)and normal control groups.The Lequesne algofunctional index was evaluated before,3 weeks after and 6 weeks after of gavage administra-tion,and the traditional Chinese medicine syndrome score was made.After 6 weeks of gavage administration,HE-stained sections of knee cartilage were prepared;the histo-morphology of knee cartilage was observed under light microscope,and modified Mankin's scores were evaluated.The ex-pression levels of proinflammatory cytokines(IL-1β and IL-6)and anti-inflammatory cytokines(IL-4 and IL-10)in sera were measured by ELISA,and the relative gene expression of MMP-1,MMP-3 and TIMP-1 in articular cartilage of KOA-YDCC rabbits was detected by RT-qPCR.Results:After 3 or 6 weeks of gavage administration,the swelling and pain re-sponse of the limbs were significantly reduced in Xishu decoction high and low dose groups;the range of motion of the knee joints and lameness were improved;the Lequesne index was significantly reduced(P＜0.05 or P＜0.01),and the traditional Chinese medicine syndrome scores were markedly decreased(P＜0.01)compared with those of the model group in the same period of time.After 6 weeks of gavage administration,compared with the model group,the modified Mankin's scores of knee cartilage in Xishu decoction high and low dose groups were significantly decreased(P＜0.01);the levels of serum IL-1β and IL-6 were significantly decreased(P＜0.01);the levels of serum IL-4 and IL-10 were markedly increased(P＜0.01);the relative gene expression of MMP-1 and MMP-3 in articular cartilage was significantly down-reg-ulated(P＜0.01),while the relative gene expression of TIMP-1 showed no significant change.Conclusion:Xishu decoction treatment could alleviate the inflammatory reaction,correct the disorder of metabolism of the joint matrix,and improve the function of the knee joint and cartilage damage in rabbits with KOA-YDCC.
Xishu decoctionknee osteoarthritissyndrome of Yang deficiency and cold coagulationproinflammatory and anti-inflammatory cytokinesmatrix metalloproteinase
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