The Study of Efficacy of the Nourishing Kidney and Invigorating Spleen Formula in Improving Osteoporotic Fracture Repair by Promoting Myogenic Differentiation
Objective:To examine the ability of the nourishing kidney and invigorating spleen formula to promote osteopo-rotic fracture healing,and to discuss the traditional Chinese medicine(TCM)theories of the relationship between spleen and kidney,the origin of the innateness and acquirement,along with disharmony between bone and muscle in the applica-tion of the osteoporotic fracture prevention.Methods:30 male C57BL/6 mice were divided into control group and nouris-hing kidney and invigorating spleen formula group,with 15 mice in each group.The nourishing kidney and invigorating spleen formula group was treated with the nourishing kidney and invigorating spleen formula,and control group was trea-ted with normal saline by gastric perfusion.The osteoporotic fracture model after castration were prepared in two groups.On the second day after fracture operation,each group was given corresponding drugs by gavage for 28 d,once every other day,and after the treatment,the mics were killed to take the samples for imaging observation,histopathological staining and reverse transcription-polymerase chain reaction(RT-PCR)and Western Blot methods to detect the expres-sion of MyoD1,MEF2C,Myf5 and Myogenin.Results:The nourishing kidney and invigorating spleen formula had an effec-tive effect in stimulating the formation of callus and improving the quality of fracture healing according to Micro-CT.The hematoxylin-eosin(HE)staining result of solus muscle showed that treatment of nourishing kidney and invigorating spleen formula could result in tighter arrangement of muscle fibers and more muscle cells than the control group.Western Blot and RT-PCR results showed that,compared with control group,the specific myogenic transcription factors of posteri-or tibial muscle,such as MyoD1,MEF2C,Myf5 and Myogenin,and protein expression in the nourishing kidney and invig-orating spleen formula group were higher,and the differences were statistically significant.Conclusion:The nourishing kid-ney and invigorating spleen formula enhanced the repair of osteoporotic fracture,and its mechanism may be related to increasing the expression of MyoD1,MEF2C,Myf5 and Myogenin in tibial attachment muscle.
osteoporosiscastrated male miceosteoporotic fracturenourishing kidney and invigorating spleen formulamyogenic differentiationtraditional Chinese medicine therapy
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