Effect of Bugan Jianyao Decoction on the Expression of MMP-3,Aggrecan Protein and mRNA in Nucleus Pulpocytes of Lumbar Disc Herniation
Objective:To explore the effect of Bugan Jianyao decoction on the expression of matrix metalloproteinase-3(MMP-3),aggrecan protein and mRNA and explore its potential targets and mechanisms of action on the treatment of lumbar intervertebral disc herniation.Methods:20 rats were randomly divided into groups to prepare Bugan Jianyao decoc-tion containing serum and normal serum;human nucleus pulposus cells(which had passed ethical review)were subjected interventions in groups.The cells were divided into 9 groups:blank group,siRNA NC group,NF-κB p65 siRNA inhibition group,5％,10％,and 20％normal serum group,and 5％,10％,and 20％serum containing the formula group.At 24 h and 48 h,RT-qPCR was used to detect the expres-sion of MMP-3,aggrecan mRNA in each group of nucleus pulposus cells,and Western Blot was used to detect the expression of MMP-3,aggrecan protein in each group.Results:In terms of group:compared with the normal serum group,the same body weight of the serum containing the formula group showed a significant decrease in MMP-3 mRNA transcription and protein expression,and showed a significant increase in aggrecan mRNA and protein expression(P＜0.05).Among the three groups of serum containing the formula,the 20％serum group showed the greatest decrease in MMP-3 mRNA transcription and protein expression,and the greatest increase in aggrecan mRNA and protein expression(P＜0.05).In terms of time:the effect of Bugan Jianyao decoction on the expression of MMP-3,aggrecan mRNA,aggrecan and MMP-3 protein were not significantly different at the two detection time points of 24 h and 48 h.Conclusion:The efficacy of Bugan Jianyao decoction is related to the dose of the medicine,and it may achieve therapeutic effects on the treatment of lumbar intervertebral disc herniation by inhibiting the NF-κB p65 signaling pathway.
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