补肝健腰方对腰椎间盘突出髓核细胞基质金属蛋白酶3、蛋白聚糖及信使核糖核酸表达的影响

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中文摘要：目的:研究补肝健腰方对基质金属蛋白酶-3(MMP-3)、蛋白聚糖(Aggrecan)蛋白及mRNA表达的影响，从而探索其治疗腰椎间盘突出症可能的疗效靶点和作用机制。方法:20只大鼠随机分组后制备补肝健腰方含药血清和正常血清，将培养的人体髓核细胞进行分组干预。实验分为9组，即空白组，siRNA NC组，NF-κB p65 siRNA阻断组，5％，10％及20％体积分数正常血清组，5％，10％及20％体积分数补肝健腰方含药血清组，在24 h和48 h时采用实时定量逆转录聚合酶链式反应(RT-qPCR)检测各组髓核细胞MMP-3、Aggrecan mRNA表达，采用蛋白质免疫印迹试验(Western Blot)检测各组MMP-3、Aggrecan蛋白表达。结果:正常血清组与补肝健腰方含药血清组比较，相同体积分数的补肝健腰方含药血清组MMP-3 mRNA转录和蛋白表达均明显降低，Aggrecan mRNA和蛋白表达明显升高(P＜0。05)，三组补肝健腰方含药血清组之间比较，以20％体积分数补肝健腰方含药血清MMP-3的mRNA转录和其蛋白表达降低最多，Aggrecan mRNA和蛋白表达提升最多(P＜0。05)。补肝健腰方对MMP-3、Aggrecan mRNA转录以及Aggrecan、MMP-3蛋白表达水平的影响在24 h和48 h两个检测时间点差异无统计学意义。结论:补肝健腰方的疗效与药物浓度有一定相关性，可能是通过抑制NF-κB p65信号通路达到治疗腰椎间盘突出症的效果。

外文标题：Effect of Bugan Jianyao Decoction on the Expression of MMP-3,Aggrecan Protein and mRNA in Nucleus Pulpocytes of Lumbar Disc Herniation

外文摘要：Objective:To explore the effect of Bugan Jianyao decoction on the expression of matrix metalloproteinase-3(MMP-3),aggrecan protein and mRNA and explore its potential targets and mechanisms of action on the treatment of lumbar intervertebral disc herniation.Methods:20 rats were randomly divided into groups to prepare Bugan Jianyao decoc-tion containing serum and normal serum;human nucleus pulposus cells(which had passed ethical review)were subjected interventions in groups.The cells were divided into 9 groups:blank group,siRNA NC group,NF-κB p65 siRNA inhibition group,5％,10％,and 20％normal serum group,and 5％,10％,and 20％serum containing the formula group.At 24 h and 48 h,RT-qPCR was used to detect the expres-sion of MMP-3,aggrecan mRNA in each group of nucleus pulposus cells,and Western Blot was used to detect the expression of MMP-3,aggrecan protein in each group.Results:In terms of group:compared with the normal serum group,the same body weight of the serum containing the formula group showed a significant decrease in MMP-3 mRNA transcription and protein expression,and showed a significant increase in aggrecan mRNA and protein expression(P＜0.05).Among the three groups of serum containing the formula,the 20％serum group showed the greatest decrease in MMP-3 mRNA transcription and protein expression,and the greatest increase in aggrecan mRNA and protein expression(P＜0.05).In terms of time:the effect of Bugan Jianyao decoction on the expression of MMP-3,aggrecan mRNA,aggrecan and MMP-3 protein were not significantly different at the two detection time points of 24 h and 48 h.Conclusion:The efficacy of Bugan Jianyao decoction is related to the dose of the medicine,and it may achieve therapeutic effects on the treatment of lumbar intervertebral disc herniation by inhibiting the NF-κB p65 signaling pathway.

外文关键词：

Bugan Jianyao decoctionlumbar disc herniationnucleusted cellsmatrix metalloproteinase-3(MMP-3)ag-grecan

作者：

郑阳、仇湘中、黄承军、陈中、张信成

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作者单位：

柳州市中医医院(广西柳州,545001)

湖南省中医药研究院附属医院

重庆市北碚区中医院

关键词：

补肝健腰方腰椎间盘突出髓核细胞基质金属蛋白酶-3 蛋白聚糖

基金：

国家中医药管理局"全国名老中医药专家仇湘中传承工作室建设项目"广西壮族自治区卫生厅中医药科技专项课题长沙市科技局项目长沙市科技局项目湖南省中医药科研计划项目

项目编号：

国中医药人教[2016]42号GZKZ-Z1106kq1901066kq1907119202021

出版年：

2024

DOI：

10.20085/j.cnki.issn1005-0205.240902

中国中医骨伤科杂志

中华中医药学会,湖北省中医药研究院

中国中医骨伤科杂志

CSTPCD

影响因子：0.732

ISSN：1005-0205

年,卷(期)：2024.32(9)