Naringin Reduces MMP-13 and Syndecan-4 in Synovial Fluid to Repair Cartilage Injury of Rabbit Knee Osteoarthritis
Objective:To investigate the effect of naringin on the repair of knee cartilage injury by reducing the concentra-tion of matrix metalloproteinase-13(MMP-13)and Syndecan-4 in synovial fluid.Methods:16 large-eared white rabbits were randomly divided into four groups:sham surgery group,model group,naringin group,and positive control group,with 4 rabbits in each group.After the establishment of the articular cartilage injury model,the model group was given deion-ized water for gastric lavage,the naringin group was given naringin for gastric lavage,and the positive control group was given glucosamine sulfate for gastric lavage.At the 12th week,the levels of MMP-13 and Syndecan-4 in the synovial fluid were detected using the enzyme-linked immunosorbent assay(ELISA)method,the repair of articular cartilage injury structure was observed by Micro-CT,and the expression level of P-Smad2 at the site of articular cartilage injury was detected by immunohistochemical method.Results:Compared with the sham surgery group,the concentrations of MMP-13 and Syndecan-4 in the synovial fluid in the model group were significantly increased(P＜0.05),indicating obvious articu-lar cartilage injury and defect,bone volume fraction,trabecu-lar separation and trabecular thickness at the site of cartilage injury were significantly lower(P＜0.05).Compared with model group,the concentrations of MMP-13 and Syndecan-4 in synovial fluid of naringin group and positive control group were significantly decreased(P＜0.05).Compared with model group,naringin group showed obvious repair performance of articular surface,and naringin group significantly improved bone volume fraction,trabecular separation and trabecular thickness at articular cartilage injury(P＜0.05).Compared with the model group,the expression level of P-Smad2 in nar-ingin group was significantly increased.Conclusion:The main active ingredient of rhizoma drynariae,naringin,can reduce the concentration levels of MMP-13 and Syndecan-4 in synovial fluid,improve the structure of articular cartilage injury in rabbit knee joints,and enhance the repair level of articular cartilage injury.Its possible mechanism is closely related to the activation of the expression levels of the TGF-β/Smad signaling pathway.Correspondingly,the inhibition of the TGF-β/Smad signaling pathway,which leads to an increase in inflammatory levels and causes articular cartilage injury,may be one of the characteristics of knee osteoarthritis with liver and kidney deficiency.
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维普
