Experimental Study on Animal Model of Spontaneous Intervertebral Disc Degeneration Induced by Postural Stress
Objective:To study the mechanism of intervertebral disc degeneration and establish an intervertebral disc degen-eration model that mimics human postural stress induction.Methods:The rat models of intervertebral disc degeneration induced by fibrous ring puncture and postural stress were establish and divide into three groups,with 10 rats in each group.Control group:No treatment,conventional feeding.Annulus fibrosus puncture(AFP)group:A model was established by puncturing the fibrous ring of the interverte-bral disc in rats using a 16th needle.Postural stress induced intervertebral disc degeneration group:Rats were placed in a moderately sized square transparent plastic tube,the tube was tilted at a 60° angle for 8 h a day,and the rats were allowed to move or rest on the inclined surface to change the stress on the rat vertebral body and establish a model.The HE staining,Masson staining,and immunohistochemical results of rats were observed at 6 and 12 weeks,respectively.Results:The rat model of intervertebral disc degeneration induced by fibrous ring puncture and postural stress was estab-lished.HE staining results showed that the number of nucleus pulposus cells decreased in the fibrous ring puncture group and the postural stress induced intervertebral disc degeneration group compared to the control group,and the decrease became more pronounced over time.The Masson staining results showed that compared with the control group,the fibrous ring structure was disrupted in the fibrous ring acupuncture group,and the boundary between the fibrous ring and the nucleus pulposus was unclear;the fibrous ring structure in the intervertebral disc degeneration group induced by postural stress was disordered,protruded significantly to both sides,and the boundary between the nucleus pulposus and the fibrous ring was unclear.The immunohistochemical results showed that compared with the control group,the number of type Ⅱ collagen positive cells in the annulus fibrosus acupuncture group and the posture stress induced intervertebral disc degeneration group decreased at week 6(P＜0.05,P＜0.01).At week 12,the annulus fibrosus acupuncture group and the posture stress induced intervertebral disc degeneration group still showed the number of type Ⅱ collagen positive cells,with a more significant decrease in the posture stress induced intervertebral disc degeneration group.However,there was no statistically significant difference between week 6 and week 12(P＜0.05,P＜0.01).The intervertebral disc degeneration score showed that compared with the control group,the fibrous ring acupuncture group and the postural stress induced intervertebral disc degeneration group had intervertebral disc degeneration at week 6(P＜0.05,P＜0.001);at week 12,the fibrous ring acupuncture group and the postural stress induced intervertebral disc degeneration group had more significant intervertebral disc degeneration(P＜0.01,P＜0.001).Conclusion:This experiment successful-ly established an intervertebral disc degeneration model through non-invasive postural stress.This model has a certain degree of innovation and can mimic the posture of human upright walking.The degeneration process is closer to the struc-ture of the human body and has a certain degree of rationality.
intervertebral disc degenerationvertebral instabilitynucleus pulposusnucleus pulposus cellsfiber ring
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