Relationship between Serum Hepatoma-Derived Growth Factor and Leukocyte Cell-Derived Chemotaxin-2 Levels with Bone Metabolism Indicators and Concurrent Osteoporosis in Patients with Rheumatoid Arthritis
Objective:To explore the relationship between serum levels of hepatoma-derived growth factor(HDGF)and leukocyte cell-derived chemotaxin-2(LECT2)with bone metabolism indicators and concurrent osteoporosis in patients with rheumatoid arthritis(RA).Methods:80 RA patients were selected as the RA group from January 2021 to December 2023.Based on the 28 joint disease activity scores(DAS28),RA patients were separated into a high RA group of 20 cases,a moderate RA group of 28 cases,and a low RA group of 32 cases.According to the presence or absence of osteoporosis,there were 50 cases in the osteoporosis group and 30 cases in the non osteoporosis group.Another 80 healthy individuals from the same period were selected as the control group.Fully automated electrochemiluminescence analyzer was applied to detect bone metabolism indicators such as procollagen type I N-terminal propeptide(PINP),25 hydroxyvitamin D(25(OH)D),β C-terminal cross-linked telopeptides of type Ⅰ collagen(β-CTX),N-terminal osteocalcin(N-MID),and bone alkaline phosphatase(B-ALP)levels.Enzyme linked immunosorbent assay(ELISA)method was applied to detect serum levels of HDGF and LECT2.Pearson method was applied to analyze correlation.Multivariate Logistic regression was applied to analyze influencing factors.Receiver operating characteristic(ROC)curve was plotted to analyze the diag-nostic value of indicators,and area under the curve(AUC)comparison was used Z-test.Results:The serum levels of HDGF,LECT2,and β-CTX in the RA group were higher than those in the control group,while the levels of PINP,25(OH)D,N-MID,and B-ALP were lower than those in the control group(P＜0,05).The serum levels of HDGF,LECT2,and β-CTX in the high RA group were higher than those in the moderate RA and low RA groups,while the levels of PINP,25(OH)D,N-MID,and B-ALP were lower than those in the moderate and low RA groups(P＜0.05).The ser-um levels of HDGF,LECT2,and β-CTX in the moderate RA group were higher than those in the low RA group,while the levels of PINP,25(OH)D,N-MID,and B-ALP in moderate RA group were lower than those in the low RA group(P＜0.05).The levels of serum HDGF and LECT2 were positively correlated with β-CTX,and negatively correlated with PINP,25(OH)D,N-MID,and B-ALP(P＜0.05).The serum levels of HDGF and LECT2 and the proportion of severe patients in the osteoporosis group were higher than those in the non osteoporosis group(P＜0.05).HDGF and LECT2 were risk factors for osteoporosis in RA patients(P＜0.05).The AUC for diagnosing osteoporosis in RA patients with serum HDGF and LECT2 were 0.847 and 0.841,respectively,and were lower than the AUC of combined diagnosis(0.927)(P＜0.05).The diagnostic thresholds for HDGF and LECT2 were 4.78 ng/mL and 34.63 ng/mL,respectively.Conclusion:The serum levels of HDGF and LECT2 in RA patients are obviously elevated,and are obviously correlated with bone metabolism indicators,and it may be associated with the occurrence of osteoporosis.When the levels of HDGF and LECT2 are higher than the diagnostic threshold,the likelihood of osteoporosis in patients is extremely high,and clini-cal physicians can take preventive medication based on their detection values.
NETL
NSTL
万方数据
