Study on the Protective Effect of Qingyi Decoction on Hepatic Ischemia-reperfusion Injury in Mice and the Mechanism of Ferroptosis
Objective:To observe the protective effect and mechanism of Qingyi Decoction(QYD)on hepatic ischemia-reperfusion injury in mice.Methods:A total of 30 male SPF C57BL/6 mice were randomly divided into 5 groups:sham,model,low-dose QYD,mid-dose QYD and high-dose QYD,with 6 mice each.Mice in QYD groups were given corresponding doses of drugs continuously 7 days before modeling,while mice in sham and mod-el groups were given same saline.The model of 70%hepatic ischemia-reperfusion injury was established by clamping the arteriovenous blood flow of left lobe and middle lobe of liver with 60 min ischemia and 12 h reperfu-sion.The following items were detected:liver enzymes by ELISA,hepatic ischemia performance by HE staining,hepatocytes'reactive oxygen species(ROS)by DHE fluorescent probe,level of ferrous ion and lipid peroxidation by enzyme-labeling measuring instrument,GPX4 expression by Western blotting and immunohistochemical stain-ing.Results:Mid-and high-dose QYD could significantly improve hepatic ischemia-reperfusion injury in mice,including effectively reducing the level of serum ALT and AST(P<0.05),the liver ischemia performance,ROS in hepatocytes,and effectively decreasing the content of ferrous ions and lipid peroxidation in liver(P<0.05).It also restored the activity of GPX4(P<0.05)as well.Conclusion:Both mid-and high-dose QYD could reduce liver oxidative stress level,hepatic ischemia performance and improve liver function.It proves that QYD has the protec-tive effect on hepatic ischemia-reperfusion injury and its potential mechanism might be related to the reduction of liver ferroptosis with a dose-dependent manner.