Objective:To investigate the antiviral effect of Shengjiang Powder on influenza virus A/PR8/34/H1N1 in vitro.Methods:MDCK cells were used as host cells and Oseltamivir phosphate as positive drug,the vi-rus titer was determined and the half tissue culture infection dose(TCID50)of influenza virus was calculated.The cytotoxicity of positive control drug and Shengjiang Powder was determined,and the maximum non-toxic concentra-tion(TC0)and half toxic concentration(TC50)were obtained.Under three different administration methods,the ef-fect of Shengjiang Powder on the cytopathic effect(CPE)of influenza A virus PR8 infection was observed in vitro.The effect of Shengjiang Powder on the cell survival rate of influenza A virus PR8 infection was calculated by CCK8 method,and the antiviral efficacy(ER)of the drug was calculated.At the same time,the expression levels of apoptosis-related factors Caspase-3,8 and 9 were dynamically detected by real-time fluorescence quantitative PCR at 12 h,24 h and 48 h after administration to explore the antiviral mechanism of Shengjiang Powder.Re-sults:The TCID50 of influenza virus PR8 was 10-520.The TC0 of oseltamivir phosphate and Shengjiang Powder were 0.25 mg/mL and 6.25 mg/mL,and the TC50 of oseltamivir phosphate and Shengjiang Powder were 0.76 mg/mL and 19.69 mg/mL.The cell survival rate of Shengjiang Powder group and oseltamivir phosphate group were higher than that of virus control group,and the difference was statistically significant(P＜0.05).Under the maxi-mum non-toxic concentration,the ER of Shengjiang Powder directly inactivated anti-influenza virus was 52.37％,which of anti-virus by preventing virus invasion and treating virus penetration into cells was less than 50％.The mRNA expressions of Caspase-3,Caspase-8 and Caspase-9 genes in Shengjiang Powder group were significantly down-regulated at 12 h,24 h and 48 h,and the difference was statistically significant(P＜0.05).Conclusion:Shengjiang Powder has a certain anti-influenza virus effect in vitro,and has inhibit effect the apoptosis induced by in-fluenza virus infection.
关键词
甲型流感病毒/升降散/体外抗病毒/细胞凋亡
Key words
Influenza A virus/Shengjiang Powder/Antiviral effect in vitro/Apoptosis
维普
万方数据