Effects of Extracts from Dabanxia Decoction on Malignant Biological Behavior and JAK1/STAT3 Pathway of Esophageal Cancer EC9706 Cells
Objective:To analyze the effects of extracts of Dabanxia Decoction extract on malignant biological behavior and JAK1/STAT3 pathway of esophageal cancer EC9706 cells,in order to provide reference for the pre-vention and treatment of esophageal cancer.Methods:Human esophageal cancer EC9706 cells were treated with Dabanxia Decoction extract(200,100,50,25,12.5 μg/mL)and STAT3 signaling pathway inhibitor(Stattic,4,3.5,3,2.5,2 μmol/L),and the cell inhibition rate was detected by MTT method.The 50％inhibitory concentration(IC50)was calculated by fitting the cell inhibition rate-concentration curve.Human esophageal cancer EC9706 cells were divided into blank control group,Dabanxia Decoction group,Stattic group and Dabanxia Decoction+Stattic group.Dabanxia Decoction group and Stattic group were treated with IC50 concentration of Dabanxia Decoc-tion extract and Stattic respectively.Dabanxia Decoction+Stattic group was treated with Dabanxia Decoction ex-tract and Stattic combined intervention.No special treatment was done in the blank control group.Cell prolifera-tion was detected by MTT assay.Cell migration ability was detected by scratch test.The invasion ability of GES-1 cells in each group was detected by Transwell assay.The mRNA and protein expression of JAK1/STAT3 signaling pathway were detected by qRT-PCR and Western blotting.Results:The inhibitory rate of EC9706 cells increased with the increase of Stattic and Dabanxia Decoction extract(P＜0.05).The results showed that the curves of Stattic and the Dabanxia Decoction extract were both excellent(R2=0.957,0.961),and their IC50 values were 98.50 µg/mL and 3.82 μmol/L,respectively.Compared with blank control group,the OD value,mobility and invasion num-ber of Dabanxia Decoction group,Stattic group and Dabanxia Decoction+Stattic group were lower(P＜0.05).Com-pared with Stattic group and Dabanxia Decoction group,the OD value,mobility and invasion number in Dabanxia Decoction+Stattic groupwere lower(P＜0.05).Compared with blank control group,the expressions of JAK1 mRNA and STAT3 mRNA in Dabanxia Decoction group,Stattic group and Dabanxia Decoction+Stattic group were lower(P＜0.05).Compared with Stattic group and Dabanxia Decoction group,the expressions of JAK1 mRNA and STAT3 mRNA in Dabanxia Decoction+Stattic group were lower(P＜0.05).Compared with blank control group,the expression levels of P-JAK1 and P-STAT3 protein in Dabanxia Decoction group and Dabanxia Decoc-tion+Stattic group were lower(P＜0.05).Compared with Dabanxia Decoction group,the p-JAK1 protein expres-sion in Dabanxia Decoction+Stattic group was lower(P＜0.05).Compared with Stattic group and Dabanxia Decoc-tion group,the p-STAT3 protein expression in Dabanxia Decoction+Stattic group was lower(P＜0.05).Conclu-sion:Dabanxia Decoction can inhibit the invasion,metastasis and proliferation of EC9706 cells,and the mecha-nism may be related to inhibiting the activation of JAK1/STAT3 pathway.
万方数据
维普
