Effect of Bushen Hemai Decoction on Hypothalamic Proteomics in Rats with Elderly Spontaneous Hypertension
Objective:To investigate the possible molecular mechanism of Bushen Hemai Decoction in the treat-ment of elderly spontaneous hypertension in rats.Methods:Male spontaneous hypertension rats(SHR)of sixteen-month-old were randomly divided into two groups:Bushen Hemai Decoction group(10 rats,with a crude drug con-centration of 14.22 g/(kg·d)and model group(10 rats,2 mL/d of normal saline),and 10 male WKY rats of the same age were used as normal control group(2 mL/d of normal saline).All received gavage for 8 weeks.The sys-tolic and diastolic blood pressure of the rats were observed and monitored regularly.After the intervention,serum and hypothalamus were collected from each group.The contents of neuropeptide Y(NPY),norepinephrine(NE),C-reactive protein(CRP)and angiotensin Ⅱ(AngⅡ)in serum were detected by ELISA.Label-free quantitative pro-teomics sequencing analysis method were used to screen the changes in hypothalamic protein expression profile,and GO enrichment analysis of differential proteins were performed by bioinformatics,the molecular function of dif-ferential proteins were analyzed,the functional aspects of cellular components and biological pathways were anno-tated,the KEGG pathway analysis of the differential proteins was carried out to obtain the target proteins of Bush-en Hemai Decoction and the signaling pathways involved,and the interaction network of differential proteins was es-tablished to comprehensively analyze the pharmacological mechanism of Bushen Hemai Decoction,so as to provide theories and ideas for the study of new drug marker proteins in the study of senile spontaneous hypertension.Re-sults:1)Bushen Hemai Decoction could steadily reduce the systolic blood pressure,diastolic blood pressure,pulse pressure difference and mean arterial pressure in rats with elderly spontaneous hypertension,and reduce the serum levels of NPY,NE,CRP and Ang Ⅱ,and improve the vascular remodeling of hypertension to a certain extent.2)Compared with the model group,a total of 2 753 differential protein points were detected in the hypothalamus of rats after the intervention of Bushen Hemai Decoction,of which 316 proteins were statistically different,201 pro-teins were increased and 115 proteins were decreased.3)The expressions of node proteins Cdc42,SIRT2 and Ptk2b in the hypothalamus of SHR were consistent with proteomics.Conclusion:The molecular mechanism of Bushen Hemai Decoction in the treatment of elderly spontaneous hypertension may be related to its promotion of neuronal cell vitality and maintenance of arterial vascular stability.
万方数据
维普
