首页|四神丸对腹泻型肠易激综合征大鼠5-HT信号通路表达的影响

四神丸对腹泻型肠易激综合征大鼠5-HT信号通路表达的影响

Effect of Sishen Pills on Expression of 5-HT Signaling Pathway in Rats with Diarrhea Irritable Bowel Syndrome

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目的 通过研究四神丸对腹泻型肠易激综合征(IBS-D)模型大鼠5-羟色胺(5-HT)信号通路表达的影响,进一步揭示其治疗IBS-D的内脏敏感机制.方法 SD大鼠32只,随机分为正常组、模型组、四神丸组和得舒特组,采用番泻叶灌胃联合避水应激法制备IBS-D大鼠模型,给药14 d后进行Bristol粪便性状量表评分,依据腹壁撤退反射评定内脏疼痛阈值,ELISA法检测血清5-HT、SERT含量,免疫组化法检测结肠组织5-HT的阳性表达,Western blotting法检测结肠组织5-HT4R的蛋白表达.结果 与正常组比较,模型组大鼠Bristol粪便性状量表评分升高,内脏疼痛阈值降低,血清中5-HT含量增加、SERT含量减少,结肠组织中5-HT的阳性表达升高、5-HT 4R的蛋白表达降低(P＜0.05或P＜0.01);与模型组比较,四神丸组大鼠Bristol粪便性状量表评分降低、内脏疼痛阈值升高,血清5-HT含量减少、SERT含量增加,结肠组织5-HT的阳性表达降低、5-HT4R的蛋白表达升高(P＜0.05或P＜0.01),且对5-HT、5-HT4R表达的影响四神丸组优于得舒特组(P＜0.05).结论 四神丸治疗IBS-D的内脏敏感机制可能与调节5-HT信号通路的表达有关.
Objective:To further reveal the mechanism of visceral sensitivity in treating diarrheal irritable bowel syndrome(IBS-D)by studying the effect of Sishen Pills on the expression of 5-hydroxytryptamine(5-HT)signaling pathway in rat model of IBS-D.Methods:A total of 32 SD rats were randomly divided into normal group,model group,Sishen Pills group and dicetel group.IBS-D rat models were prepared by senna leaf gavage combined with water stress avoidance method.After 14 days of administration,bristol fecal trait scale score was performed,and visceral pain threshold was assessed according to abdominal wall withdrawal reflex.The content of 5-HT and SERT in serum was detected by ELISA,the positive expression of 5-HT in colon tissue was detected by immunohistochemistry,and the protein expression of 5-HT4R in colon tissue was detected by Western blotting.Results:Compared with normal group,the score of Bristol fecal trait scale of the model group was increased,visceral pain threshold was decreased,serum 5-HT content was increased,SERT content was decreased,positive expression of 5-HT was increased and protein expression of 5-HT4R was decreased(P＜0.05 or P＜0.01).Com-pared with model group,the scores of Bristol fecal trait scale decreased and visceral pain threshold increased,serum 5-HT content decreased and SERT content increased in Sishen Pills group,the positive expression of 5-HT in colon tissues was decreased and the protein expression of 5-HT4R was increased(P＜0.05 or P＜0.01),and the Sishen Pills group was better than dicetel group in the expression of 5-HT and 5-HT4R(P＜0.05).Conclusion:The mechanism of visceral sensitivity of IBS-D treated by Sishen Pills may be related to regulating the expression of 5-HT signaling pathway.

Diarrhea irritable bowel syndromeSishen Pills5-HT signaling pathwayVisceral sensitivityRats

魏星旭、易梦妮、邓娜、蔺晓源、周赛男

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湖南中医药大学,湖南长沙 410208

湖南中医药大学第一附属医院,湖南长沙 410007

腹泻型肠易激综合征 四神丸 5-HT信号通路 内脏敏感性 大鼠

湖南省自然科学基金湖南省教育厅科学研究项目湖南省教育厅科学研究项目湖南中医药大学中医学国内一流建设学科开放基金湖南省大学生创新创业训练计划

2020JJ543221B036821C02212021ZYX15S202310541011

2024

10.3969/j.issn.1004-745X.2024.02.006

中国中医急症
中华中医药学会

中国中医急症

CSTPCD
影响因子：1.144
ISSN：1004-745X
年,卷(期)：2024.33(2)
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