Benefits of Atractylon via Nrf2-NLRP3 Signaling Pathway Modulation on Mice with LPS-induced Acute Lung Injury
Objective:To investigate the protective effect of atractylon on LPS-induced acute lung injury(ALl)in mice by regulating Nrf2-NLRP3 signaling pathway.Methods:A mouse model of ALI was established.The mice were divided into the control group,model group,dexamethasone group,and atractylon low,middle,high dose group.The levels of TNF-α,IL-1β,1L-6 and IL-10 in serum and bronchoalveolar lavage fluid(BALF)were measured by ELISA.The wet/dry weight ratio(W/D)of lung tissue was measured to evaluate the degree of edema.The activities of MPO,GSH-Px,SOD and MDA in lung tissue were detected to evaluate the anti-oxidative damage in vivo.The lung injury was evaluated by HE staining and scored.RT-qPCR was used to measure the mRNA lev-els of Nrf2,HO-1,NLRP3,ASC,caspase-1,IL-1β,and IL-18 in lung tissue of mice in each group.Results:Atractylon showed a good protective effect on LPS-induced ALI in mice,which reduced the levels of TNF-α,IL-1β,IL-6 and W/D ratio in serum and alveolar lavage fluid,MPO,MDA in lung tissue and the mRNA levels of NL-RP3,ASC,caspase-1,IL-1β,IL-18;increased the level of IL-10 in serum and the levels of GSH-Px,SOD in lung tissue,the mRNA levels of Nrf2 and HO-1;the pathological manifestations of lung tissue were improved,and the pathological scores of lung tissue were decreased in a dose-dependent manner.Conclusion:Atraetylon can al-leviate lung injury in ALI mice by regulating inflammatory factors and oxidative stress,which may be related to the regulation of Nrf2-NLRP3 pathway.
万方数据
维普
