The Effect of Tongxie Anchang Decoction on Short-chain Fatty Acids Metabolism and Th1/Th2 Cell Immune Balance in Mice with Irritable Bowel Syndrome
Objective:To investigate the modulating effect of Tongxie Anchang Decoction on short-chain fatty acids metabolism and Th1/Th2 cell immune balance in mice of irritable bowel syndrome with predominant diarrhea(IBS-D)with syndrome of stagnation of liver qi and spleen deficiency.Methods:30 male C57BL/6 mice,weigh-ing 18～22 g,were randomly divided into control group,model group,Tongxie Anchang Decoction low group,Tongx-ie Anchang Decoction medium group,and Tongxie Anchang Decoction high group,with 6 mice in each group.The IBS-D mouse model was replicated using restraint stress combined with senna leaf gavage.After successful model-ing,corresponding dose of Tongxie Anchang Decoction were administered by gavage once a day for 14 days.The changes in short chain-fatty acid content in the feces of mice were detected by targeted metabolomics.The propor-tion of CD3+CD4+INF-γ+T cells(Th1 cells)and CD3+CD4+IL-4+T cells(Th2 cells)in mesenteric lymph nodes were detected by flow cytometry.Results:Compared with the control group,the relative content of fecal short-chain fatty acids(SCFAs)of mice in the model group increased(P＜0.01),with butyric acid,propionic acid and acetic acid all significantly increased(P＜0.01 or P＜0.05).After treatment with the different dose of Tongxie An-chang Decoction,the relative content of total SCFAs in feces decreased(P＜0.01),while butyric acid,propionic ac-id and acetic acid all decreased to varying degrees.Among them,each dose group of Tongxie Anchang Decoction significantly reduced the content of acetic acid and butyric acid(P＜0.01).The low dose and medium dose groups significantly reduced the content of propionic acid(P＜0.01).In the model group,the proportion of Th2 cells in mouse mesenteric lymph nodes significantly decreased,while the proportion of Th1 cells and Th1/Th2 significantly increased,with a statistically significant difference(P＜0.05);after treatment,the proportion of Th2 cells in each dose group of Tongxie Anchang Decoction increased,while the proportion of Th1 cells and Th1/Th2 decreased.Conclusion:Tongxie Anchang Decoction can reduce the content of SCFAs in the feces of IBS-D mice with syn-drome of stagnation of liver qi and spleen deficiency,and regulate Th1/Th2 cell immune disorders in mice.
万方数据
维普
