Mechanism of Dahuang Mudan Decoction in Treating Gastrointestinal Dysfunction in Sepsis
Objective:To explore the potential molecular mechanism of Dahuang Mudan Decoction in treating gastrointestinal dysfunction in sepsis through network pharmacology.Methods:The chemical components of Da-huang Mudan Decoction were obtained and screened from TCMSP,TCMID and TCM Database@Taiwan database,and the drug targets were retrieved using Swiss Target Prediction database;Genecards was used to screen the dis-ease-related action sites of sepsis and gastrointestinal dysfunction;the intersection genes of drug action targets and disease-related action sites were obtained by Venny 2.1.0 online tool,and the network diagram of"traditional Chi-nese medicine-compounds-potential therapeutic targets"with multi-level interaction was drawn by combining string 12.0 database and Cytoscape 3.9.1 software;David database was used to complete the GO and KEGG path-way enrichment analysis of potential therapeutic targets.Results:A total of 53 chemical components and 479 ac-tion targets of Dahuang Mudan Decoction were obtained,and 124 potential therapeutic targets were obtained after intersection with 2 121 sepsis-related action sites and 6 785 gastrointestinal dysfunction-related action sites.The key targets of Dahuang Mudan Decoction in treating gastrointestinal dysfunction in sepsis were IL-6,IL-1β,Aktl,etc.Through enrichment analysis,it was found that Dahuang Mudan Decoction in treating gastrointestinal dysfunc-tion in sepsis played an anti-inflammatory and immune system regulating role by regulating HIF-1 signaling path-way,PI3K-Akt signaling pathway,IL-17 signaling pathway,etc.Conclusions:Through the network pharmacology method,we found that the mechanism of Dahuang Mudan Decoction in treating gastrointestinal dysfunction in sep-sis is complex,involving multiple components,multiple targets and multiple pathways,which may be related to an-ti-inflammatory,immune system,etc.
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