Protective Effect of Tanshinone ⅡA on Pulmonary Microvascular Endothelial Cell Injury in Sepsis
Objective:To explore the protective effect of Tanshinone Ⅱ A(Tan Ⅱ A)on endothelial cell injury in septic pulmonary vasculature.Methods:A mouse model of septic lung injury was constructed using cecal ligation and puncture(CLP).Mice were divided into six groups:sham operation group,model group,experimental group 1[CLP+10 mg/kg Tan ⅡA],experimental group 2[CLP+30 mg/kg Tan Ⅱ A],experimental group 3[CLP+50 mg/kg Tan Ⅱ A],and experimental group 4[CLP+100 mg/kg Tan Ⅱ A].After 24 hours of treatment,various indicators such as lung tissue pathology,pulmonary microvascular permeability,lung tissue wet/dry weight ratio,white blood cell count,and inflammatory factors were measured.The sham group underwent only cecal exploration.Results:The experimental groups showed reduced lung tissue injury and scores compared with the model group(P＜0.05).Compared with the model group,the experimental groups exhibited significantly lower Evans Blue content and lung tissue wet/dry weight ratio(P＜0.05).Compared with the model group,the experimental groups had signifi-candy reduced white blood cell count,protein content,and inflammatory factors in bronchoalveolar lavage fluid,with statistically significant differences(all P＜0.05).There were no significant differences in lung tissue pathologi-cal damage,Evans Blue content,lung tissue wet/dry weight ratio,and the expression levels of IL-6,TNF-α,IL-1β,and other inflammatory factors in bronchoalveolar lavage fluid among experimental group 2,experimental group 3,and experimental group 4(all P＞0.05).Conclusion:Tan ⅡA can dose-dependently reduce endothelial cell inju-ry in septic pulmonary microvasculature,thus improving lung injury,and 30 mg/kg Tan ⅡA can achieve the best ef-fect.
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