调心安神针法对缺血再灌注心律失常大鼠心肌组织微小RNA的影响
Effect of Tiaoxin Anshen Acupuncture on MicroRNAs of Myocardial Tissue of Ischemia-Reperfusion Arrhythmic Rats
郑渝凡 1李明妍 1徐敏 1崔华峰 1曹龙 2彭文东 2郭丽 1王锐1
作者信息
- 1. 山东中医药大学附属医院,山东济南 250014
- 2. 山东中医药大学,山东济南 250014
- 折叠
摘要
目的 观察调心安神针法对缺血再灌注心律失常(RA)大鼠心肌组织微小RNA(miRNA1、miR-NA133a)表达的影响以了解调心安神针法治疗缺血再灌注心律失常的作用机制.方法 40只大鼠随机分为假手术组、模型组、针刺组及胺碘酮组,针刺组施以调心安神针刺法预处理7 d(取穴:灵台、神道、双侧内关、百会).采用结扎/松解左冠状动脉前降支的方法制作RA模型大鼠.造模完毕后,酶联免疫法检测血清肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)含量,苏木精-伊红染色观察心肌组织,RT-PCR法检测缺血区中央部位心内膜心肌miRNA1、miRNA133a的表达水平.结果 针刺组、胺碘酮组CK-MB和LDH的含量较模型组明显降低(P＜0.01).针刺组、胺碘酮组miRNA1表达均较模型组明显降低(P＜0.01),且胺碘酮组低于针刺组(P＜0.01);针刺组和胺碘酮组miRNA133a表达均较模型组明显增加(P＜0.05),针刺组与胺磺酮组差异无统计学意义(P＞0.05).结论 调心安神针法预处理和注射胺碘酮均能改善心肌损伤,减少心律失常的发生,其机制可能与通过下调缺血再灌注心律失常大鼠心肌组织miRNA1表达,上调miRNA133a的表达有关.
Abstract
Objective:To observe the effect of Tiaoxin Anshen acupuncture on the expression of miRNA(miR-NAl,miRNA133a)in myocardium of ischemia reperfusion arrhythmia(RA)model rats,and to explore the mecha-nism of ischemia reperfusion arrhythmia.Methods:RA model rats were made by ligating and loosening the anteri-or descending branch of the left coronary artery.40 Rats were divided into sham operation group,model group,acu-puncture group and amiodarone group.The acupuncture group were given Tiaoxin Anshen acupuncture pretreat-ment for 7 days at Lingtai(GV10),Shendao(GV11),bilateral Neiguan(PC6)and Baihui(GV20).After the experi-ment,ELISA method was used to detect serum CK-MB and LDH content,and HE staining was used to observe myocardial tissue,RT-PCR method was used to detect the expression levels of miRNA1 and miRNA133a in the endocardial myocardium in the central part of the ischemic area.Results:The contents of CK-MB and LDH in acupuncture group and amiodarone group were significantly lower than those in model group(P＜0.01).The ex-pression of miRNA1 in acupuncture group and amiodarone group was significantly lower than that in model group(P＜0.01),and amiodarone group was lower than that in acupuncture group(P＜0.01).The expression of miR-NA133a in acupuncture group and amiodarone group was significantly increased compared with model group(P＜0.05),but there was no difference between the two groups(P＞0.05).Conclusion:Tiaoxin Anshen acupuncture pretreatment and amiodarone injection could improve myocardial damage and reduce the occurrence of ischemia-reperfusion arrhythmias,and the mechanism may be related to the downregulation of miRNA1 and upregulation of miRNA133a in myocardial tissue of ischemia-reperfusion rats.
关键词
缺血再灌注性心律失常/针刺/调心安神针法/微小RNA1/微小RNA133a大鼠Key words
Ischemia reperfusion arrhythmia/Acupuncture/Tiaoxin Anshen acupuncture/miRNA1/miR-NA133a/Rats引用本文复制引用
基金项目
山东省中医药科技发展计划(2019-0092)
山东省自然科学基金面上项目(ZR2021MH312)
出版年
2024
NETL
NSTL
万方数据