Effects of Jiawei Duhuo Jisheng Mixture on Serum IL-6,IL-1β,TNF-α and Synovial Inflammation in Mice with Knee Osteoarthritis
Objective:To explore the effect of Jiawei Duhuo Jisheng Mixture on serum IL-6,IL-1β and TNF-αin mice with knee osteoarthritis,and to preliminary determinate the optimal dosage of Jiawei Duhuo Jisheng Mix-ture for the treatment of knee osteoarthritis.Methods:A total of 36 C57/BL6j mice were divided into a blank group,a model group,a control group,and a low,medium,and high-dose group of Jiawei Duhuo Jisheng Mixture.Except for the blank group,knee osteoarthritis models were established by injecting papain solution into the knee joint.The blank group and model group were given physiological saline by gavage,while the control group was giv-en diacerein capsule solution by gavage.The low,medium,and high-dose groups of Jiawei Duhuo Jisheng Mixture were given 6.28 g/kg,12.56 g/kg,25.12 g/kg by gavage.After continuous gavage for 8 weeks,mice in each group were scored on the modified Lequesen MG scale,and serum IL-6,IL-1β and TNF-α were measured by ELISA;HE staining and Masson staining were used to observe the structural morphology and fibrous tissue proliferation of the knee joint synovial membrane.Results:The improved Lequesen MG scale score in the model group was signif-icantly increased(P＜0.01),and the content of serum inflammatory factors was significantly increased(P＜0.01).HE staining showed abundant vascular proliferation and infiltration of inflammatory cells in synovial tissue;Mas-son staining showed a large amount of collagen fiber proliferation;the modified Lequesen MG scale scores of the control group and the low-dose group with modified Duhuo Jisheng Mixture were significantly lower than the mod-el group(P＜0.01),and the content of serum inflammatory factors was significantly reduced(P＜0.01).HE stain-ing showed improvement in synovitis,while Masson staining showed a reduction in collagen fiber proliferation;the mid-dose and high-dose groups of Jiawei Duhuo Jisheng Mixture showed a significant decrease in the score of the modified Lequesen MG scale(P＜0.01),and a significant decrease in serum inflammatory factor content(P＜0.01).HE staining showed a significant improvement in synovitis inflammation,while Masson staining showed a signifi-cant reduction in collagen fiber proliferation.Conclusion:Jiawei Duhuo Jisheng Mixture can reduce serum IL-6,IL-1β and TNF-α levels in mice with knee osteoarthritis,improve the content of synovial inflammation in mice knee joints.The dosage of 12.56 g/kg of Jiawei Duhuo Jisheng Mixture showed the most significant improvement.
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