银黄含片对H1N1流感小鼠炎症因子表达的影响研究
Effect of Yinhuang Buccal Tablets on Inflammatory Factors Expression in Mice with H1N1 Influenza
苏右竹 1苏芮 1吴彩军 2连博 1刘密凤 1范杰 1王烁 1刘清泉1
作者信息
- 1. 首都医科大学北京中医医院,北京 100010
- 2. 北京中医药大学东直门医院,北京 100700
- 折叠
摘要
目的 评价银黄含片对H1N1流感小鼠的药效,观察其对H1N1流感小鼠炎症因子表达水平的影响.方法 采用流感病毒H1N1CA07滴鼻建立H1N1小鼠模型,奥司他韦组与银黄含片组分别予奥司他韦及银黄含片溶液灌胃7 d.观察小鼠肺指数及病毒指数.采用高通量液相蛋白芯片检测小鼠血清IFN-γ、IL-12p70、IL-17A、IP-10、MIP-1β、ENA-78.结果 银黄含片组肺指数较模型组显著降低,模型组小鼠IL-12p70、ENA-78和IL-17A水明显降低(P＜0.01),IP-10明显增高(P＜0.01).银黄含片组IFN-γ水平与MIP-1β水平较奥司他韦组均明显降低P＜0.05).结论 银黄含片能够明显减少H1N1小鼠的肺损伤,并明显降低IFN-γ与MIP-1β水平,推测银黄含片很可能是通过减少T细胞的趋化,降低流感小鼠的Th l免疫应答,从而起到达到肺保护作用.
Abstract
Objective:To evaluate the efficacy of Yinhuang Buccal Tablet on mice with H1N1 influenza and to explore its effect on inflammatory factors in H1N1 influenza mice.Methods:H1N1 mouse model was established by nasal drip of influenza virus H1N1CA07.The control group and the observation group were given oseltamivir and Yinhuang Buccal Tablet solution respectively by gavage for 7 days.The changes of inflammatory factors includ-ing IFN-γ,IL-12p70,IL-17A,IP-10,M1P-1β and ENA-78 in serum of mice were detected by high throughput liquid phase protein chip.Results:The diseased lung index of Yinhuang Buccal Tablet group was significantly lower than that of model group.The IL-12p70,ENA-78 and IL-17A water in model group were significantly lower(P＜0.01),and the IP-10 was significantly higher(P＜0.01).IFN-y and MIP-1β levels in the Yinhuang Buccal Tablet group were significantly lower than those of oseltamivir group(P＜0.05).Conclusion:Yinhuang Buccal Tablet could significantly reduce lung injury in H1N1 mice and the levels of IFN-y and MIP-1β.It might reduce the Th1 immune response of influenza mice by reducing the chemotaxis of T cells,thus achieving lung protection.
关键词
流感/银黄含片/炎症因子/免疫应答/小鼠Key words
Influenza/Yinhuang Buccal Tablet/Inflammatory factors/Immune response/Mice引用本文复制引用
基金项目
国家中医药局高水平中医药重点学科建设项目(zyyzdxk-2023001)
首都卫生发展科研专项(2020-2-4192)
出版年
2024
NETL
NSTL
万方数据