首页|肺复方通过PI3K/Akt/Nrf2信号通路对A549/DDP细胞耐药性的影响

肺复方通过PI3K/Akt/Nrf2信号通路对A549/DDP细胞耐药性的影响

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目的 观察肺复方对人肺癌顺铂耐药株A549/DDP细胞耐药性的作用及对PI3K/Akt/Nrf2信号通路的影响.方法 选用A549/DDP细胞设立不含药血清的空白对照组、肺复方组、顺铂组、联合组,流式细胞术检测各组细胞周期分布和细胞中活性氧(ROS)的变化,Western blotting和RT-PCR检测PI3K/Akt/Nrf2信号通路及下游血红素氧合酶1(HO-1)、NAD(P)H:醌氧化还原酶1(NQO1)、多药耐药相关蛋白1(MRP1)的表达变化.结果 与空白对照组、顺铂组相比,联合组引起细胞G1期阻滞,增加细胞内活性氧的累积,差异具有统计学意义(P＜0.05或P＜0.01);与空白对照组比,肺复方组和联合组能减少Nrf2核转位,下调p-Akt、HO-1、NQ01、MRP1蛋白表达和mRNA表达水平,差异具有统计学意义(P＜0.05或P＜0.01).结论 肺复方能够通过调控PI3K/Akt/Nrf2信号通路增加A549/DDP细胞对顺铂的敏感性.
Effect of Feifu Decoction on Drug Resistance in A549/DDP Cells Through PI3K/Akt/Nrf2 Signaling Pathway
Objective:To investigate the effect of Feifu Decoction combined with cisplatin on chemotherapy sen-sitization of lung cancer A549/DDP cells and its effect on PI3K/Akt/Nrf2 signal pathway.Methods:A549/DDP cells were selected to establish drug-free serum control group,Feifu Decoction group,cisplatin group and com-bined group.The cell cycle distribution and the changes of reactive oxygen species(ROS)in cells were detected by flow cytometry.Western blotting and RT-PCR were used to detect the expression of PI3K/Akt/Nrf2 signal path-way and downstream heme oxygenase-1(HO-1),NAD(P)h:quinone oxidoreductase 1(NQO1),multidrug resis-tance associated protein 1(MRP1).Results:Compared with the control group and cisplatin group,the combined group caused G1 phase arrest and increased the accumulation of intracellular ROS,which was statistically signifi-cant(P＜0.05 or P＜0.01).Compared with the control group,Feifu Decoction group and combined group could re-duce Nrf2 nuclear translocation and down-regulate the protein expression and mRNA expression levels of p-Akt,HO-1,NQO1 and MRP1,which was statistically significant(P＜0.05 or P＜0.01).Conclusion:Feifu Decoction can increase the sensitivity of A549/DDP cells to cisplatin by regulating PI3K/Akt/Nrf2 signaling pathway.

Lung cancerFeifu DecoctionA549/DDP cellsPI3K/Akt/Nrf2 signaling pathwayDrug resistance

谭小宁、梁子成、柳卓、邱云

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湖南省中医药研究院附属医院,中医肿瘤学湖南省重点实验室,湖南省中西医结合肿瘤临床研究中心,湖南长沙 410006

肺癌 肺复方 A549/DDP细胞 PI3K/Akt/Nrf2信号通路 耐药性

湖南省重点研发计划湖南省自然科学基金湖南省中医药计划湖南省中医药计划湖南省卫生健康委项目湖南省卫生健康委项目长沙市自然科学基金全国中药特色技术传承人才培训项目湖南省中医药骨干人才项目(十四五)

2023SK20572022JJ402382021173D2022064202113051181202203105606kq2202478国中医药人教函[2023]96号湘中医药[2024]3号

2024

10.3969/j.issn.1004-745X.2024.06.005

中国中医急症
中华中医药学会

中国中医急症

CSTPCD
影响因子：1.144
ISSN：1004-745X
年,卷(期)：2024.33(6)
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