摘要
目的 通过观察三子利水颗粒对心衰肺水肿大鼠肺组织病理组织学、AQP1、AQP5、ENaC蛋白及mRNA表达的影响探讨其治疗心衰肺水肿的机制.方法 选择雄性SD大鼠,通过结扎腹主动脉、腹腔注射氯化铵造成心衰急性肺水肿模型,随机分为模型组、西药组、中药低剂量组、中药高剂量组、中药低剂量+西药组、中药高剂量+西药组.给药1周后观察各组大鼠肺组织含水率、病理组织学及AQP1、AQP5、ENaC蛋白及mRNA表达水平.结果 与模型组比较,各给药组肺含水量均减少(P＜0.05);组织细胞间的炎性浸润和肺泡间的充血症状均改善.AQP1、AQP5蛋白表达均不同程度地升高,中药低剂量组AQP1差异有统计学意义(P＜0.05),AQP5差异无统计学意义(P＞0.05),其余给药组差异有统计学意义(P＜0.01).ENaC蛋白表达均不同程度地升高,空白对照组、中药低剂量组+西药组、中药高剂量组+西药组差异有统计学意义(P＜0.05),其余各组差异无统计学意义(P＞0.05).AQP1、AQP5mRNA表达均不同程度地升高,中药低剂量组+西药组、中药高剂量组+西药组差异有统计学意义(P＜0.01).ENaC mRNA表达均不同程度地升高,中药低剂量组+西药组差异有统计学意义(P＜0.05),西药组、中药高剂量组+西药组差异有统计学意义(P＜0.01).中药低、高剂量组差异无统计学意义(P＞0.05).结论 三子利水颗粒可通过升高AQP1、AQP5、ENaC蛋白表达及mRNA表达含量来改善上皮细胞病理组织形态,达到清上焦肺水,缓解心衰肺水肿体征的目的.
Abstract
Objective:To explore the mechanism of Sanzi Lishui Granules in the treatment of heart failure and pulmonary edema by observing its effects on the pathological histology,AQP1,AQP5,ENaC protein and mRNA ex-pression in lung tissue of rats with heart failure and pulmonary edema.Methods:Male SD rats were selected and randomly divided into the model group,western medicine control group,Chinese medicine low dose group,Chinese medicine high dose group,Chinese medicine low dose+western medicine group,Chinese medicine high dose+west-ern medicine group by ligating the abdominal aorta and injecting ammonium chloride intraperitoneally to create a model of heart failure and acute pulmonary edema.Changes in water content,pathological histology and expression of AQP1,AQP5,ENaC protein and mRNA in lung tissue of rats in each group were observed after 1 week of ad-ministration.Results:Compared with the model group,the lung water content of each treatment group decreased(P＜0.05);The inflammatory infiltration between tissue cells and congestion symptoms between alveoli have both improved.The expression of AQP1 and AQP5 proteins increased to varying degrees.The low-dose group of tradi-tional Chinese medicine showed statistically significant differences in AQP1(P＜0.05),while there was no statisti-cally significant difference in AQP5(P＞0.05).The other treatment groups showed statistically significant differenc-es(P＜0.01).The expression of ENaC protein increased to varying degrees,with significant differences observed in the blank control group,low-dose Chinese medicine group+western medicine group,and high-dose Chinese medi-cine group+western medicine group(P＜0.05),while no significant differences were observed in the other groups(P＞0.05).The expression of AQP1 and AQP5mRNA increased to varying degrees,and there was a statistically significant difference between the low-dose group of traditional Chinese medicine and the western medicine group,as well as the high-dose group of traditional Chinese medicine and the western medicine group(P＜0.01).The ex-pression of ENaC mRNA increased to varying degrees,and there was a statistically significant difference between the low-dose group of traditional Chinese medicine and the western medicine group(P＜0.05).There was a statisti-cally significant difference between the western medicine group,the high-dose group of traditional Chinese medi-cine,and the western medicine group(P＜0.01).There was no statistically significant difference between the low-dose and high-dose groups of traditional Chinese medicine(P＞0.05).Conclusion:Sanzi Lishui Granules can im-prove the pathological histomorphology of epithelial cells by elevating the protein expression and mRNA expres-sion content of AQP1,AQP5,and ENaC,and achieve the purpose of clearing the upper warmer lung water,thus al-leviating the signs of heart failure and pulmonary edema.
基金项目
吉林省科技厅自然科学研究项目(20210101225JC)
NETL
NSTL
万方数据