Effects of Sanzi Lishui Granules on the Expression of Fluid Transport-Related Proteins in Lung Tissue of Rats with Heart Failure and Pulmonary Edema Based on the Theory of"Lung Regulates Water Channels"
Objective:To explore the mechanism of Sanzi Lishui Granules in the treatment of heart failure and pulmonary edema by observing its effects on the pathological histology,AQP1,AQP5,ENaC protein and mRNA ex-pression in lung tissue of rats with heart failure and pulmonary edema.Methods:Male SD rats were selected and randomly divided into the model group,western medicine control group,Chinese medicine low dose group,Chinese medicine high dose group,Chinese medicine low dose+western medicine group,Chinese medicine high dose+west-ern medicine group by ligating the abdominal aorta and injecting ammonium chloride intraperitoneally to create a model of heart failure and acute pulmonary edema.Changes in water content,pathological histology and expression of AQP1,AQP5,ENaC protein and mRNA in lung tissue of rats in each group were observed after 1 week of ad-ministration.Results:Compared with the model group,the lung water content of each treatment group decreased(P＜0.05);The inflammatory infiltration between tissue cells and congestion symptoms between alveoli have both improved.The expression of AQP1 and AQP5 proteins increased to varying degrees.The low-dose group of tradi-tional Chinese medicine showed statistically significant differences in AQP1(P＜0.05),while there was no statisti-cally significant difference in AQP5(P＞0.05).The other treatment groups showed statistically significant differenc-es(P＜0.01).The expression of ENaC protein increased to varying degrees,with significant differences observed in the blank control group,low-dose Chinese medicine group+western medicine group,and high-dose Chinese medi-cine group+western medicine group(P＜0.05),while no significant differences were observed in the other groups(P＞0.05).The expression of AQP1 and AQP5mRNA increased to varying degrees,and there was a statistically significant difference between the low-dose group of traditional Chinese medicine and the western medicine group,as well as the high-dose group of traditional Chinese medicine and the western medicine group(P＜0.01).The ex-pression of ENaC mRNA increased to varying degrees,and there was a statistically significant difference between the low-dose group of traditional Chinese medicine and the western medicine group(P＜0.05).There was a statisti-cally significant difference between the western medicine group,the high-dose group of traditional Chinese medi-cine,and the western medicine group(P＜0.01).There was no statistically significant difference between the low-dose and high-dose groups of traditional Chinese medicine(P＞0.05).Conclusion:Sanzi Lishui Granules can im-prove the pathological histomorphology of epithelial cells by elevating the protein expression and mRNA expres-sion content of AQP1,AQP5,and ENaC,and achieve the purpose of clearing the upper warmer lung water,thus al-leviating the signs of heart failure and pulmonary edema.
Pulmonary edemaSanzi Lishui GranulesAquaporin 1Aquaporin5Sodium channels in epithelial cellsRats
NETL
NSTL
万方数据
