大柴胡汤通过TLR4/NF-KB通路改善重症中暑大鼠炎症的机制研究
Study on the Mechanism of Dachaihu Decoction in Improving Inflammation in Rats with Severe Heat Stroke Through the TLR 4/NF-κB Pathway
陈冬杰 1黄永莲 1彭晓洪 1黄亚秀 1黄发根 1张爱婷 1王评1
作者信息
- 1. 北京中医药大学深圳医院(龙岗),广东深圳 518000
- 折叠
摘要
目的 观察大柴胡汤对重症中暑大鼠炎症的治疗效果并探寻其作用机制.方法 SPF级SD雄性大鼠60只随机分为空白对照组、模型组、中药低剂量组、中药高剂量组.将大鼠置于高温气候动物培养箱内[温度(40.50±0.50)℃,湿度(65.00±2.00)％]制备经典中暑模型,模型制备成功后6 h开始灌胃给药,8 h 1次,连续2d.在造模成功后6、24、48 h各处死5只大鼠,检测血清白细胞介素-1(IL-1)、肿瘤坏死因子-α(TNF-α),同时取回肠组织,采用蛋白质免疫印迹试验测定肠道组织中TLR4、核转录因子-κB(NF-κB)蛋白表达.结果 与空白对照组比较,模型组、中药低剂量组和中药高剂量组各时间点血清IL-1、TNF-α水平和肠道组织中TLR4、NF-κB蛋白表达水平均升高(P＜0.05或P＜0.01);中药低、高剂量组造模后24、48 h血清TNF-α、IL-1水平和肠道组织中TLR4、NF-κB的蛋白表达水平均较模型组显著下降(P＜0.05).中药高剂量组TNF-α、TLR4、NF-κB蛋白表达水平在造模后24、48 h较低剂量组明显下降,而IL-1水平在造模后48 h明显下降(P＜0.05).结论 大柴胡汤能抑制TLR4/NF-KB信号通路,减轻肠道炎症反应,从而起到保护重症中暑大鼠的作用.
Abstract
Objective:To investigate the effect of Dachaihu Decoction in improving inflammation in rats with se-vere heat stroke and to investigate the mechanism of action.Methods:A total of 60 male rats were randomly di-vided into the blank control group,model group,standard dose traditional chinese medicine group and high dose traditional chinese medicine group.The classic heat stroke model was prepared by placing rats in a high tempera-ture climate animal incubator[temperature(40.50±0.50)℃,humidity(65.00±2.00)％].After successful prepara-tion,the model was administered by gavage q8h at 6 h for 2 consecutive days.5 rats were killed at 6 h,24 h and 48 h after successful modeling,and interleukin-1(IL-1),tumor necrosis factor-α(TNF-α),and ileum tissue were collected.The protein expression of TLR4 and NF-κB in intestinal tissues was determined by protein immunoblot-ting test.Results:Compared with the blank control group,the serum levels of IL-1 and TNF-α and the expres-sion levels of TLR4 and NF-κB proteins in intestinal tissues were increased in the model group,the low-dose group and the high-dose traditional chinese medicine group(P＜0.05 or P＜0.01).The serum levels of TNF-αand IL-1 and the protein expression levels of TLR4 and NF-κB in intestinal tissues were significantly decreased at 24 and 48 h after the model group(P＜0.05).The protein expression levels of TNF-α,TLR4 and NF-κB in the high-dose traditional chinese medicine group decreased significantly at 24 and 48 h after modeling,while the lev-els of IL-1 decreased significantly at 48 h after modeling(P＜0.05).Conclusion:Dachaihu Decoction can inhibit TLR 4/NF-κB signaling pathway,reduce intestinal inflammation and gastrointestinal damage,and play a role in protecting the rats with severe heat stroke.
关键词
重症中暑/大柴胡汤/TLR4/NF-KB通路/炎症反应/大鼠Key words
Severe heat stroke/Dachaihu Decoction/TLR4/NF-κB pathway/Inflammatory response/Rats引用本文复制引用
基金项目
广东省深圳市龙岗区医药卫生科技计划(LGKCYLWS2020072)
广东省深圳市龙岗区科技创新专项医疗卫生科技计划(LGWJ2022-68)
出版年
2024
NETL
NSTL
万方数据