Effect of Dunhuang Xintong Formula on Myocardial Enzymes,Oxidative Stress,and Serum Biochemical Markers in Rats with Myocardial Infarction
Objective:To observe the effects of Dunhuang Xintong Formula(Xiaoshi Xionghuang Powder)on myocardial enzymes,oxidative stress,and serum biochemical markers in rats with myocardial infarction.Methods:Wistar rats were randomly divided into a control group,model group,aspirin group,nitroglycerin group,and Xiaoshi Xionghuang Powder group.Except for the control group,all other groups were subjected to acute myocardi-al ischemia induced by isoproterenol.The Xiaoshi Xionghuang Powder group received Xiaoshi Xionghuang Powder paste application on the Zhiyang acupoint(DU9),the nitroglycerin group received nitroglycerin patch,and the aspi-rin group received aspirin enteric-coated tablets.The model group and control group received an equal amount of saline orally.One hour after intervention,HE staining was used to observe pathological changes in myocardial tis-sue.Colorimetric method was used to evaluate the effects of interventions on myocardial enzyme profiles(CK,AST,LDH),oxidative stress markers(SOD,NOS),and serum levels of HMG-CoA reductase,vasoconstrictor(ET),and vasodilator(NO)factors.ELISA and nitrate reductase method were used to measure the differences in serum levels of HMG-CoA reductase,ET,and NO.Results:Compared with the control group,the model group showed signifi-cant increases in serum levels of CK,AST,LDH,ET,NOS,and HMG-CoA reductase(P＜0.05).Compared with the model group,the aspirin group,nitroglycerin group,and Xiaoshi Xionghuang Powder group showed significant decreases in serum levels of AST,LDH,ET,NOS,and HMG-CoA reductase(P＜0.05).The aspirin group and Xiaoshi Xionghuang Powder group exhibited significant decreases in serum CK activity compared with the model group(P＜0.05).Compared with the aspirin group,the nitroglycerin group showed lower serum LDH levels(P＜0.05)and higher ET and NOS levels(P＜0.05).The Xiaoshi Xionghuang Powder group had significantly lower se-rum CK and AST levels compared with the nitroglycerin group(P＜0.05).Conclusion:Xiaoshi Xionghuang Pow-der may attenuate myocardial ischemic necrosis,improve endothelial function,reduce the release of myocardial en-zymes,and downregulate NOS for vasodilation,thereby improving myocardial infarction.
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