Effect of Yujiang Paidu Decoction on NF-κB Signaling Pathway of Acute Rhinosinusitis Rats
Objective:To investigate the effect of Yujiang Paidu Decoction on the expression of NF-κB path-way-associated proteins in acute rhinosinusitis rats.Methods:The rat model of acute rhinosinusitis was estab-lished through unilateral nasal inoculation with Staphylococcus aureus.The effectiveness of the model was as-sessed based on the severity scale for acute rhinosinusitis.Rats were divided into the normal,model,Yujiang Pai-du Decoction and positive drug(Amoxicillin clarithromycin)groups.The pH value of nasal secretions in each group was determined.HE staining was employed to observe the histopathological alterations of nasal mucosa.The levels of TNF-α,IL-6,and IL-1 β in nasal lavage fluid and nasal mucosal supernatant were detected by ELI-SA.Rats with acute rhinosinusitis were subjected to Western blotting analysis to analyze the effect of Yujiang Pai-du Decoction on the expression levels of NF-κB signaling pathway-related proteins.Results:The acute rhinosi-nusitis rat inflammation model was successfully established.Compared with the model group,the pH value of the Yujiang Paidu Decoction and the positive drug group was significantly reduced(P＜0.01),the pathological injury of nasal mucosa was significantly improved,the levels of TNF-α,IL-1 β,and IL-6 in nasal lavage fluid and nasal mucosa supernatant were significantly decreased(P＜0.01)and the expression level of NF-κB related protein was significantly decreased(P＜0.01).Conclusion:Yujiang Paidu Decoction can ameliorate the symptoms of acute rhinosinusitis by suppressing the expression of NF-κB related signaling proteins in rats with acute rhinosi-nusitis.
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