Exploring the Effect of Butuo Fumigation on Postoperative Wound Healing of Perianal Abscess in Rats Using Label-free based Quantitative Proteomics Analysis
Objective:To observe the effect of Butuo fumigation on postoperative wound healing in rats with perianal abscess,and to explore the possible mechanism of action by label-free proteomic analysis.Methods:A postoperative wound model of perianal abscess was established using Specific Pathogen Free(SPF)Sprague-Daw-ley(SD)rats.The rats were randomly divided into three groups:the control group,the model group,and the Butuo fumigation group.After continuous treatment for 14 days,the growth of the wounds was observed.Hematoxylin-eo-sin(HE)staining was used to observe the histopathological changes in the wound tissues.Label-free proteomics analysis was employed to identify differential proteins in the postoperative wound tissues from the control group,model group,and the Butuo fumigation group.Results:Compared with the control group and the model group,the collagen formation and epidermal growth of the Butuo fumigation group were better(P＜0.05).The proteomics analysis revealed 11 differentially expressed proteins among the three groups.Tartrate-resistant Acid Phosphatase 5(TRAP/ACP5)was present in all three groups;notably,ACP5 levels in the Butuo fumigation group showed a downward trend compared with the model and control groups.Conclusion:Butuo fumigation can inhibit inflamma-tory factors and promote the postoperative wound healing of perianal abscess in rats by regulating the expression of p38/JNK MAPK signaling pathway.
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